The notion of directly introducing new genes or otherwise directly manipulating the genotype
of an animal is conceptually straightforward and appealing because of the speed and …

Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene
(neo) and a human coagulation factor IX genomic construct designed for expression of the …

The ability to directly manipulate the pig genome through genetic engineering has been
available to the research community for over three decades. This technology has …

Infection of the mammary gland, in addition to causing animal distress, is a major economic
burden of the dairy industry. Staphylococcus aureus is the major contagious mastitis …

Background Transgenic animals have become valuable tools for both research and applied
purposes. The current method of gene transfer, microinjection, which is widely used in …

Ejaculated sperm cells from both bovine and swine animals depleted of the seminal fluid
were used as vectors for transferring different plasmid DNAs into eggs during the fertilization …

The implementation of large-scale genome mapping and sequencing has improved the
understanding of animal genetics. A large number of gene sequences are now available to …

During the past 20 years, transgenesis has become a popular technique and a crucial tool
for molecular geneticists and biologists. Transgene expression is now better-controlled and …

Selection of transgenic embryos prior to embryo transfer is a means to increase the
efficiency of transgenic livestock production. Among transgenic reporters, cytoplasmic …

Transgenic animal technology is one of the most fascinating technologies developed in the
last two decades. It allows us to address questions in life sciences that no other methods …