Analysis and purification of synthetic oligonucleotides by reversed-phase high-performance liquid chromatography with photodiode array and mass spectrometry …

M Gilar - Analytical biochemistry, 2001 - Elsevier
Native and modified synthetic oligonucleotides were purified by reversed-phase HPLC
using volatile ion-pairing mobile phases. Purification of 10–90 nmol of oligonucleotides in a …

A genotyping system capable of simultaneously analyzing> 1000 single nucleotide polymorphisms in a haploid genome

HY Wang, M Luo, IV Tereshchenko, DM Frikker… - Genome …, 2005 - genome.cshlp.org
A high-throughput genotyping system for scoring single nucleotide polymorphisms (SNPs)
has been developed. With this system,> 1000 SNPs can be analyzed in a single assay, with …

Serotyping of Toxoplasma gondii infections in Humans Using Synthetic Peptides

JT Kong, ME Grigg, L Uyetake… - The Journal of …, 2003 - academic.oup.com
To determine whether the characteristics of disease due to Toxoplasma gondii
(toxoplasmosis) are dependent on the infecting strain, we have developed an enzyme …

Design of base-discriminating fluorescent nucleoside and its application to T/C SNP typing

A Okamoto, K Tanaka, T Fukuta… - Journal of the American …, 2003 - ACS Publications
We report a novel method for base detection using a base-discriminating fluorescent (BDF)
nucleoside. We developed BDF probes containing methoxybenzodeazaadenine MDA and …

Fluorescent microsphere‐based readout technology for multiplexed human single nucleotide polymorphism analysis and bacterial identification

F Ye, MS Li, JD Taylor, Q Nguyen, HM Colton… - Human …, 2001 - Wiley Online Library
Large‐scale human genotyping requires technologies with a minimal number of steps, high
accuracy, and the ability to automate at a reasonable cost. In this regard, we have …

Direct transduction of allele-specific primer extension into electrical signal using genetic field effect transistor

T Sakata, Y Miyahara - Biosensors and Bioelectronics, 2007 - Elsevier
We have developed a genetic field effect transistor (FET) for single nucleotide polymorphism
(SNP) genotyping, which is based on potentiometric detection of molecular recognition on …

Validation of the high-throughput marker technology DArT using the model plant Arabidopsis thaliana

AHJ Wittenberg, T Lee, C Cayla, A Kilian… - Molecular Genetics and …, 2005 - Springer
Abstract Diversity Arrays Technology (DArT) is a microarray-based DNA marker technique
for genome-wide discovery and genotyping of genetic variation. DArT allows simultaneous …

Whole‐genome genotyping

KL Gunderson, FJ Steemers, H Ren, P Ng, L Zhou… - Methods in …, 2006 - Elsevier
We have developed an array‐based whole‐genome genotyping (WGG) assay (Infinium)
using our BeadChip platform that effectively enables unlimited multiplexing and …

Genetic approaches to studying virulence and pathogenesis in Toxoplasma gondii

J Blackwell, C Newbold, M Turner… - … Transactions of the …, 2002 - royalsocietypublishing.org
Toxoplasma gondii is a common protozoan parasite that causes disease in
immunocompromised humans. Equipped with a wide array of experimental tools, T. gondii …

Snipping polymorphisms from large EST collections in barley (Hordeum vulgare L.)

R Kota, S Rudd, A Facius, G Kolesov, T Thiel… - Molecular Genetics and …, 2003 - Springer
The public EST (expressed sequence tag) databases represent an enormous but
heterogeneous repository of sequences, including many from a broad selection of plant …