Groundbreaking advances in volume electron microscopy and specimen preparation are
enabling the 3-dimensional visualisation of specimens with unprecedented detail, and …

Electrophysiology, the'gold standard'for investigating neuronal signalling, is being
challenged by a new generation of optical probes. Together with new forms of microscopy …

Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-
neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only …

A key challenge in neuroscience is the expeditious reconstruction of neuronal circuits. For
model systems such as Drosophila and C. elegans, the limiting step is no longer the …

Animal behaviour arises from computations in neuronal circuits, but our understanding of
these computations has been frustrated by the lack of detailed synaptic connection maps, or …

Optical methods for viewing neuronal populations and projections in the intact mammalian
brain are needed, but light scattering prevents imaging deep into brain structures. We …

The bold and thrilling quest to finally understand the brain We know that each of us is
unique, but science has struggled to pinpoint where, precisely, our uniqueness resides. Is it …

The balance between excitatory and inhibitory (E and I) synapses is thought to be critical for
information processing in neural circuits. However, little is known about the spatial principles …

In the cerebral cortex, local circuits consist of tens of thousands of neurons, each of which
makes thousands of synaptic connections. Perhaps the biggest impediment to …

The last decade has seen a rapid increase in the number of tools to acquire volume electron
microscopy (EM) data. Several new scanning EM (SEM) imaging methods have emerged …