Abstract Polyadenylation [poly (A)] signals (PAS) are a defining feature of eukaryotic protein-
coding genes. The central sequence motif AAUAAA was identified in the mid-1970s and …

Formation of mRNA 3′ ends in eukaryotes requires the interaction of transacting factors
with cis-acting signal elements on the RNA precursor by two distinct mechanisms, one for …

Transcription of the mammalian genome is pervasive, but productive transcription outside of
protein-coding genes is limited by unknown mechanisms. In particular, although RNA …

Recombinant proteins, such as monoclonal antibodies, are produced in mammalian cell
lines to introduce proper protein folding and post‐translational modifications, which are …

Most eukaryotic mRNA precursors (premRNAs) must undergo extensive processing,
including cleavage and polyadenylation at the 3′-end. Processing at the 3′-end is …

Pre‐mRNA cleavage and polyadenylation is an essential step for 3′ end formation of
almost all protein‐coding transcripts in eukaryotes. The reaction, involving cleavage of …

We constructed and analyzed a synthetic poly (A)(SPA) site that was based on the highly
efficient poly (A) signal of the rabbit beta-globin gene. By use of the SPA, we demonstrate …

The physiological relevance of structures within mammalian mRNAs has been elusive, as
these mRNAs are less folded in cells than in vitro and have predicted secondary structures …

X-linked chronic granulomatous disease is an immunodeficiency characterized by defective
production of microbicidal reactive oxygen species (ROS) by phagocytes. Causative …

The termination of transcription of eukaryotic genes by RNA polymerase H, at first sight,
appears to be a rather random process, with the heterogeneous transcripts produced being …