Precise plant genome editing using base editors and prime editors
The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
CRISPR/Cas system: recent advances and future prospects for genome editing
H Manghwar, K Lindsey, X Zhang, S Jin - Trends in plant science, 2019 - cell.com
Genome editing (GE) has revolutionized biological research through the new ability to
precisely edit the genomes of living organisms. In recent years, various GE tools have been …
precisely edit the genomes of living organisms. In recent years, various GE tools have been …
The emerging and uncultivated potential of CRISPR technology in plant science
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
PAM-less plant genome editing using a CRISPR–SpRY toolbox
Q Ren, S Sretenovic, S Liu, X Tang, L Huang, Y He… - Nature plants, 2021 - nature.com
The rapid development of the CRISPR–Cas9,–Cas12a and–Cas12b genome editing
systems has greatly fuelled basic and translational plant research,,,,–. DNA targeting by …
systems has greatly fuelled basic and translational plant research,,,,–. DNA targeting by …
CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement
Since it was first recognized in bacteria and archaea as a mechanism for innate viral
immunity in the early 2010s, clustered regularly interspaced short palindromic repeats …
immunity in the early 2010s, clustered regularly interspaced short palindromic repeats …
CRISPR–Act3. 0 for highly efficient multiplexed gene activation in plants
RNA-guided CRISPR activation (CRISPRa) systems have been developed in plants.
However, the simultaneous activation of multiple genes remains challenging. Here, we …
However, the simultaneous activation of multiple genes remains challenging. Here, we …
Precision genome editing using cytosine and adenine base editors in mammalian cells
Genome editing has transformed the life sciences and has exciting prospects for use in
treating genetic diseases. Our laboratory developed base editing to enable precise and …
treating genetic diseases. Our laboratory developed base editing to enable precise and …
Base-editing-mediated artificial evolution of OsALS1 in planta to develop novel herbicide-tolerant rice germplasms
Y Kuang, S Li, B Ren, F Yan, C Spetz, X Li, X Zhou… - Molecular plant, 2020 - cell.com
Recently developed CRISPR-mediated base editors, which enable the generation of
numerous nucleotide changes in target genomic regions, have been widely adopted for …
numerous nucleotide changes in target genomic regions, have been widely adopted for …
[HTML][HTML] Construct design for CRISPR/Cas-based genome editing in plants
CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …