Chromis-1, a ratiometric fluorescent probe optimized for two-photon microscopy reveals dynamic changes in labile Zn (II) in differentiating oligodendrocytes

D Bourassa, CM Elitt, AM McCallum… - ACS …, 2018 - ACS Publications
Despite the significant advantages of two-photon excitation microscopy (TPEM) over
traditional confocal fluorescence microscopy in live-cell imaging applications, including …

[HTML][HTML] Non-cell-autonomous regulation of optic nerve regeneration by amacrine cells

EG Sergeeva, PA Rosenberg… - Frontiers in Cellular …, 2021 - frontiersin.org
Visual information is conveyed from the eye to the brain through the axons of retinal
ganglion cells (RGCs) that course through the optic nerve and synapse onto neurons in …

The oxidative balance orchestrates the main keystones of the functional activity of cardiomyocytes

M Bevere, C Morabito, MA Mariggiò… - … Medicine and Cellular …, 2022 - Wiley Online Library
This review is aimed at providing an overview of the key hallmarks of cardiomyocytes in
physiological and pathological conditions. The main feature of cardiac tissue is the force …

Interactions of zinc aqua complexes with ovalbumin at the forefront of the Zn2+/ZnO-OVO hybrid complex formation mechanism

B Buszewski, P Žuvela, A Król-Górniak… - Applied Surface …, 2021 - Elsevier
The present research is focused on experimental and computational mechanistic study of
the nanocomplexes formation through Zn 2+(aq)-ovalbumin (OVA) binding. The …

Zinc as allosteric ion channel modulator: ionotropic receptors as metalloproteins

FA Peralta, JP Huidobro-Toro - International Journal of Molecular …, 2016 - mdpi.com
Zinc is an essential metal to life. This transition metal is a structural component of many
proteins and is actively involved in the catalytic activity of cell enzymes. In either case, these …

Dissecting the process of activation of cancer-promoting zinc-requiring ectoenzymes by zinc metalation mediated by ZNT transporters

T Tsuji, Y Kurokawa, J Chiche, J Pouysségur… - Journal of Biological …, 2017 - ASBMB
Zinc-requiring ectoenzymes, including both secreted and membrane-bound enzymes, are
considered to capture zinc in their active site for their activation in the early secretory …

Inside-out design of zinc-binding proteins with non-native backbones

SL Guffy, SV Pulavarti, J Harrison, D Fleming… - Biochemistry, 2023 - ACS Publications
The de novo design of functional proteins requires specification of tertiary structure and
incorporation of molecular binding sites. Here, we develop an inside-out design strategy in …

Increased free Zn2+ correlates induction of sarco(endo)plasmic reticulum stress via altered expression levels of Zn2+‐transporters in heart failure

Y Olgar, A Durak, E Tuncay, CV Bitirim… - Journal of Cellular …, 2018 - Wiley Online Library
Zn2+‐homoeostasis including free Zn2+ ([Zn2+] i) is regulated through Zn2+‐transporters
and their comprehensive understanding may be important due to their contributions to …

How structural biology has directly impacted our understanding of P2X receptor function and gating

SE Mansoor - The P2X7 Receptor: Methods and Protocols, 2022 - Springer
Abstract P2X receptors are ATP-gated ion channels expressed in a wide variety of
eukaryotic cells. They play key roles in diverse processes such as platelet activation, smooth …

Activation of zinc-requiring ectoenzymes by ZnT transporters during the secretory process: Biochemical and molecular aspects

T Kambe, T Takeda, Y Nishito - Archives of biochemistry and biophysics, 2016 - Elsevier
In humans, about 1000 enzymes are estimated to bind zinc. In most of these enzymes, zinc
is present at the active site; thus, these enzymes are functional as “zinc-requiring enzymes” …