The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …

The ever-expanding set of CRISPR technologies and their programmable RNA-guided
nucleases exhibit remarkable flexibility in DNA targeting. However, this flexibility comes with …

Base editing—the introduction of single-nucleotide variants (SNVs) into DNA or RNA in
living cells—is one of the most recent advances in the field of genome editing. As around …

Genome-wide association studies (GWAS) have successfully mapped thousands of loci
associated with complex traits. These associations could reveal the molecular mechanisms …

Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …

RNA-guided programmable nucleases from CRISPR systems generate precise breaks in
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …

According to Darwin's theory, endless evolution leads to a revolution. One such example is
the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)–Cas system, an …

The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein
9 (CRISPR/Cas9) gene editing technology, as a revolutionary breakthrough in genetic …

Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems are well-
known acquired immunity systems that are widespread in archaea and bacteria. The RNA …

The ability to modify multiple genetic elements simultaneously would help to elucidate and
control the gene interactions and networks underlying complex cellular functions. However …