Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria
Transcriptional regulation by nuclease-deficient CRISPR/Cas is a popular and valuable tool
for routine control of gene expression. CRISPR interference in bacteria can be reliably …
for routine control of gene expression. CRISPR interference in bacteria can be reliably …
Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design
B Farboud, BJ Meyer - Genetics, 2015 - academic.oup.com
Success with genome editing by the RNA-programmed nuclease Cas9 has been limited by
the inability to predict effective guide RNAs and DNA target sites. Not all guide RNAs have …
the inability to predict effective guide RNAs and DNA target sites. Not all guide RNAs have …
The CRISPR–Cas system for plant genome editing: advances and opportunities
Genome editing is an approach in which a specific target DNA sequence of the genome is
altered by adding, removing, or replacing DNA bases. Artificially engineered hybrid …
altered by adding, removing, or replacing DNA bases. Artificially engineered hybrid …
CRISPRi-seq for genome-wide fitness quantification in bacteria
CRISPR interference (CRISPRi) is a powerful tool to link essential and nonessential genes
to specific phenotypes and to explore their functions. Here we describe a protocol for …
to specific phenotypes and to explore their functions. Here we describe a protocol for …
In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
B Álvarez, M Mencía, V De Lorenzo… - Nature …, 2020 - nature.com
In vivo mutagenesis systems accelerate directed protein evolution but often show restricted
capabilities and deleterious off-site mutations on cells. To overcome these limitations, here …
capabilities and deleterious off-site mutations on cells. To overcome these limitations, here …
Applications of CRISPR/Cas system to bacterial metabolic engineering
The clustered regularly interspaced short palindromic repeats/CRISPR-associated
(CRISPR/Cas) adaptive immune system has been extensively used for gene editing …
(CRISPR/Cas) adaptive immune system has been extensively used for gene editing …
Development of a CRISPR-Cas9 tool kit for comprehensive engineering of Bacillus subtilis
AW Westbrook, M Moo-Young… - Applied and …, 2016 - Am Soc Microbiol
The establishment of a clustered regularly interspaced short palindromic repeat (CRISPR)-
Cas9 system for strain construction in Bacillus subtilis is essential for its progression toward …
Cas9 system for strain construction in Bacillus subtilis is essential for its progression toward …
CRISPR-RNAa: targeted activation of translation using dCas13 fusions to translation initiation factors
Tools for synthetically controlling gene expression are a cornerstone of genetic engineering.
CRISPRi and CRISPRa technologies have been applied extensively for programmable …
CRISPRi and CRISPRa technologies have been applied extensively for programmable …
Modified polynucleotides for the production of biologics and proteins associated with human disease
S Bancel, T Chakraborty, A De Fougerolles… - US Patent …, 2015 - Google Patents
First worldwide family litigation filed litigation Critical https://patents. darts-ip. com/? family=
49235350&utm_source= google_patent&utm_medium= platform_link&utm_campaign …
49235350&utm_source= google_patent&utm_medium= platform_link&utm_campaign …
Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution
Y Wu, A Jameel, XH Xing, C Zhang - Trends in biotechnology, 2022 - cell.com
Adaptive laboratory evolution (ALE) has served as a historic microbial engineering method
that mimics natural selection to obtain desired microbes. The past decade has witnessed …
that mimics natural selection to obtain desired microbes. The past decade has witnessed …