Engineered dCas9 with reduced toxicity in bacteria: implications for genetic circuit design
Large synthetic genetic circuits require the simultaneous expression of many regulators.
Deactivated Cas9 (dCas9) can serve as a repressor by having a small guide RNA (sgRNA) …
Deactivated Cas9 (dCas9) can serve as a repressor by having a small guide RNA (sgRNA) …
[HTML][HTML] A navigation guide of synthetic biology tools for Pseudomonas putida
Pseudomonas putida is a microbial chassis of huge potential for industrial and
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …
[PDF][PDF] A CRISPR/Cas9-based system for reprogramming cell lineage specification
Gene activation by the CRISPR/Cas9 system has the potential to enable new approaches to
science and medicine, but the technology must be enhanced to robustly control cell …
science and medicine, but the technology must be enhanced to robustly control cell …
[HTML][HTML] CRISPR–Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains
V Stovicek, I Borodina, J Forster - Metabolic Engineering Communications, 2015 - Elsevier
There is a demand to develop 3rd generation biorefineries that integrate energy production
with the production of higher value chemicals from renewable feedstocks. Here, robust and …
with the production of higher value chemicals from renewable feedstocks. Here, robust and …
[PDF][PDF] Conditionally stabilized dCas9 activator for controlling gene expression in human cell reprogramming and differentiation
D Balboa, J Weltner, S Eurola, R Trokovic… - Stem cell reports, 2015 - cell.com
CRISPR/Cas9 protein fused to transactivation domains can be used to control gene
expression in human cells. In this study, we demonstrate that a dCas9 fusion with repeats of …
expression in human cells. In this study, we demonstrate that a dCas9 fusion with repeats of …
In vivo production of proteins
S Bancel, T Chakraborty, A De Fougerolles… - US Patent …, 2015 - Google Patents
2013-03-19 Assigned to modeRNA Therapeutics reassignment modeRNA Therapeutics
ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors …
ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors …
In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
B Álvarez, M Mencía, V De Lorenzo… - Nature …, 2020 - nature.com
In vivo mutagenesis systems accelerate directed protein evolution but often show restricted
capabilities and deleterious off-site mutations on cells. To overcome these limitations, here …
capabilities and deleterious off-site mutations on cells. To overcome these limitations, here …
CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002
GC Gordon, TC Korosh, JC Cameron, AL Markley… - Metabolic …, 2016 - Elsevier
Trans-acting regulators provide novel opportunities to study essential genes and regulate
metabolic pathways. We have adapted the clustered regularly interspersed palindromic …
metabolic pathways. We have adapted the clustered regularly interspersed palindromic …
Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
T Chakraborty, A De Fougerolles - US Patent 9,303,079, 2016 - Google Patents
2013-03-19 Assigned to modeRNA Therapeutics reassignment modeRNA Therapeutics
ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors …
ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors …
CRISPR-RNAa: targeted activation of translation using dCas13 fusions to translation initiation factors
Tools for synthetically controlling gene expression are a cornerstone of genetic engineering.
CRISPRi and CRISPRa technologies have been applied extensively for programmable …
CRISPRi and CRISPRa technologies have been applied extensively for programmable …