High-throughput genetic screens are powerful methods to identify genes linked to a given
phenotype. The catalytic null mutant of the Cas9 RNA-guided nuclease (dCas9) can be …

Genome‐wide screens have discovered a large set of essential genes in the opportunistic
human pathogen Streptococcus pneumoniae. However, the functions of many essential …

Bacteria from the same species can differ widely in their gene content. In Escherichia coli,
the set of genes shared by all strains, known as the core genome, represents about half the …

The EUROFUNG network is a virtual centre of multidisciplinary expertise in the field of fungal
biotechnology. The first academic-industry Think Tank was hosted by EUROFUNG to …

Transcriptional regulation is central to the complex behavior of natural biological systems
and synthetic gene circuits. Platforms for the scalable, tunable, and simple modulation of …

Corynebacterium glutamicum is an important organism for the industrial production of amino
acids. Metabolic pathways in this organism are usually engineered by conventional methods …

Clustered, regularly interspaced, short palindromic repeats–CRISPR associated (CRISPR-
Cas) systems are prokaryotic RNA-directed endonuclease machineries that act as an …

CRISPR-Cas systems offer versatile technologies for genome engineering, yet their
implementation has been outpaced by ongoing discoveries of new Cas nucleases and anti …

Plant annexins are Ca 2+-dependent phospholipid-binding proteins and exist as multigene
families in plants. They are implicated in the regulation of plant development as well as …

CRISPR-Cas9 technology has rapidly changed the landscape for how biologists and
bioengineers study and manipulate the genome. Derived from the bacterial adaptive …