Microbial cell factories (MCFs) convert low-cost carbon sources into valuable compounds.
The CRISPR/Cas9 system has revolutionized MCF construction as a remarkable genome …

Rice leaf morphology is an essential agronomic trait to develop drought-tolerant genotypes
for adequate and stable crop production in drought-prone areas. Here, rolled leaf mutant …

Genetic circuits require many regulatory parts in order to implement signal processing or
execute algorithms in cells. A potentially scalable approach is to use dC as9, which employs …

The identification of the trans-acting factors and cis-regulatory modules that are involved in
human pluripotent stem cell (hPSC) maintenance and differentiation is necessary to dissect …

CRISPR-Cas systems have shown tremendous promise as heterologous tools for genome
editing and transcriptional regulation. Because these RNA-directed immune systems are …

Synthetic biology seeks to create new biological systems, including user-designed plants
and plant cells. These systems can be employed for a variety of purposes, ranging from …

We describe the application of clustered regularly interspaced short palindromic repeats
interference (CRISPRi) for gene repression in the model cyanobacterium Synechcocystis sp …

The CRISPR‐Cas9 system has transformed genome engineering of model organisms from
possible to practical. CRISPR‐Cas9 can be readily programmed to generate sequence …

The clustered regularly interspaced short palindromic repeats (CRISPR)/dCas9 system has
been widely applied in both transcriptional regulation and epigenetic studies. However, for …

One of the key drivers for successful metabolic engineering in microbes is the efficacy by
which genomes can be edited. As such there are many methods to choose from when …