[HTML][HTML] Intestinal 'infant-type'bifidobacteria mediate immune system development in the first 1000 days of life

C Lin, Y Lin, H Zhang, G Wang, J Zhao, H Zhang… - Nutrients, 2022 - mdpi.com
Immune system maturation begins early in life, but few studies have examined how early-life
gut microbiota colonization educates the neonatal immune system. Bifidobacteria …

Recent progress on health effects and biosynthesis of two key sialylated human milk oligosaccharides, 3′-sialyllactose and 6′-sialyllactose

Y Zhu, J Zhang, W Zhang, W Mu - Biotechnology Advances, 2023 - Elsevier
Human milk oligosaccharides (HMOs), the third major solid component in breast milk, are
recognized as the first prebiotics for health benefits in infants. Sialylated HMOs are an …

De novo biosynthesis of 2′-fucosyllactose in a metabolically engineered Escherichia coli using a novel ɑ1, 2-fucosyltransferase from Azospirillum lipoferum

Y Chen, Y Zhu, H Wang, R Chen, Y Liu, W Zhang… - Bioresource …, 2023 - Elsevier
Human milk oligosaccharides are complex, indigestible oligosaccharides that provide ideal
nutrition for infant development. Here, 2′-fucosyllactose was efficiently produced in …

Strategies for enhancing microbial production of 2′-fucosyllactose, the most abundant human milk oligosaccharide

Y Liu, Y Zhu, H Wang, L Wan, W Zhang… - Journal of Agricultural …, 2022 - ACS Publications
Human milk oligosaccharides (HMOs), a group of structurally diverse unconjugated glycans
in breast milk, act as important prebiotics and have plenty of unique health effects for …

Biosynthesis of human milk oligosaccharides: enzyme cascade and metabolic engineering approaches

J Meng, Y Zhu, H Wang, H Cao… - Journal of Agricultural and …, 2023 - ACS Publications
Human milk oligosaccharides (HMOs) have unique beneficial effects for infants and are
considered as the new gold standard for premium infant formula. They are a collection of …

Utilization of glycosyltransferases as a seamless tool for synthesis and modification of the oligosaccharides-A review

MY Ali, F Liaqat, MI Khazi, S Sethupathy… - International Journal of …, 2023 - Elsevier
Glycosyltransferases (GTs) catalyze the transfer of active monosaccharide donors to
carbohydrates to create a wide range of oligosaccharide structures. GTs display strong …

Engineering Escherichia coli for high-level production of lacto-N-fucopentaose I by stepwise de novo pathway construction

Z Li, Y Zhu, Z Huang, P Zhang, W Zhang, W Mu - Carbohydrate Polymers, 2023 - Elsevier
Abstract Lacto-N-fucopentaose I (LNFP I) is an abundant and important fucosylated human
milk oligosaccharide (HMO). Here, an efficient LNFP I-producing strain without by-product …

Elimination of byproduct generation and enhancement of 2′-fucosyllactose synthesis by expressing a novel α1, 2-fucosyltransferase in engineered Escherichia coli

Y Zhu, R Chen, H Wang, Y Chen, Y Liu… - Journal of Agricultural …, 2023 - ACS Publications
2′-Fucosyllactose (2′-FL) is a kind of fucosylated human milk oligosaccharide (HMO),
representing the most abundant oligosaccharide in breast milk. We conducted systematic …

Pathway Optimization and Uridine 5′-Triphosphate Regeneration for Enhancing Lacto-N-Tetraose Biosynthesis in Engineered Escherichia coli

Z Li, Y Zhu, P Zhang, W Zhang… - Journal of Agricultural and …, 2022 - ACS Publications
Recently, human milk oligosaccharides (HMOs) have attracted increasing attention and
display great commercial importance, especially for the infant formula industry. Lacto-N …

Efficient Production of a Functional Human Milk Oligosaccharide 3′-Sialyllactose in Genetically Engineered Escherichia coli

J Zhang, Y Zhu, W Zhang, W Mu - ACS Synthetic Biology, 2022 - ACS Publications
3′-Sialyllactose (3′-SL) is one of the most important and simplest sialylated human milk
oligosaccharides. In this study, a plasmid-based pathway optimization along with …