Enzymatic reduction of CO 2 through cascade reactions into useful chemicals or fuels is a
promising option to reduce CO 2 emission. However, the temperature of exhaust flue gas …

The structure of formate dehydrogenase from Candida boidinii (CbFDH) is of both academic
and practical interests. First, this enzyme represents a unique model system for studies on …

NAD+-dependent formate dehydrogenase (FDH) from Candida boidinii was cloned and
expressed to a high level in Escherichia coli (20% of soluble E. coli protein). Molecular …

Isotope substitution of enzymes has become a means of addressing the participation of
protein motions in enzyme-catalyzed reactions. The idea is that only the enzyme mass will …

Isotopically labeled enzymes (denoted as “heavy” or “Born–Oppenheimer” enzymes) have
been used to test the role of protein dynamics in catalysis. The original idea was that the …

The 2′, 3′‐dialdehyde derivative of ADP (oADP) has been shown to be an affinity label for
the NAD+ binding site of recombinant Candida boidinii formate dehydrogenase (FDH) …

辅酶的再生与反复利用对氧化还原型生物催化剂在手性化合物中的生产具有重要作用.
辅酶的酶催化法再生具有高效, 专一的特点, 根据其催化环境可分为单细胞内辅酶再生体系 …

Formaldehyde and methylformate affect the growth of Candida boidinii on methanol and the
activity of methanol-degrading enzymes. The presence of both intermediates in the feeding …

Experiments on Vibrational Energy Transfer (VET) in proteins contribute to our
understanding of fundamental biological processes such as allostery, dissipation of excess …

Cloning and expression of the L-phenylalanine dehydrogenase gene, from B. sphaericus in
E. coli were done. The gene was cloned in the vector pET16b and transformed into E. coli …