The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer
bacterium to become a flexible and engineer-able platform for metabolic engineering stems …

Biotechnological production in bacteria enables access to numerous valuable chemical
compounds. Nowadays, advanced molecular genetic toolsets, enzyme engineering as well …

Background Plasmids are widely used for molecular cloning or production of proteins in
laboratory and industrial settings. Constant modification has brought forth countless plasmid …

Population heterogeneity is omnipresent in all bioprocesses even in homogenous
environments. Its origin, however, is only so well understood that potential strategies like bet …

Microbial bioprocessing based on orthologous pathways constitutes a promising approach
to replace traditional greenhouse gas-and energy-intensive production processes, eg, for …

A systematic and powerful knowledge‐based framework exists for improving the activity and
stability of chemical catalysts and for empowering the commercialization of respective …

Phenotypic plasticity of microbial cells has attracted much attention and several research
efforts have been dedicated to the description of methods aiming at characterizing …

Understanding the composability of genetic elements is central to synthetic biology. Even for
seemingly well-known elements such as a sigma 70 promoter the genetic context …

Ribosomal RNA (rRNA) operons have recently been identified as promising sites for
chromosomal integration of genetic elements in Pseudomonas putida, a bacterium that has …

High gene expression of enzymes partaking in recombinant production pathways is a
desirable trait among cell factories belonging to all different kingdoms of life. High enzyme …