[HTML][HTML] Sheep and goat genome engineering: from random transgenesis to the CRISPR era

P Kalds, S Zhou, B Cai, J Liu, Y Wang… - Frontiers in …, 2019 - frontiersin.org
Sheep and goats are valuable livestock species that have been raised for their production of
meat, milk, fiber, and other by-products. Due to their suitable size, short gestation period …

[HTML][HTML] Extranuclear inheritance of mitochondrial genome and epigenetic reprogrammability of chromosomal telomeres in somatic cell cloning of mammals

M Samiec, M Skrzyszowska - International Journal of Molecular Sciences, 2021 - mdpi.com
The effectiveness of somatic cell nuclear transfer (SCNT) in mammals seems to be still
characterized by the disappointingly low rates of cloned embryos, fetuses, and progeny …

[HTML][HTML] Structural biology and protein engineering of thrombolytics

J Mican, M Toul, D Bednar, J Damborsky - Computational and structural …, 2019 - Elsevier
Myocardial infarction and ischemic stroke are the most frequent causes of death or disability
worldwide. Due to their ability to dissolve blood clots, the thrombolytics are frequently used …

Use of genome editing techniques to produce transgenic farm animals

AN Hay, K Farrell, CM Leeth, K Lee - Recent Advances in Animal Nutrition …, 2022 - Springer
Recombinant proteins are essential for the treatment and diagnosis of clinical human
ailments. The availability and biological activity of recombinant proteins is heavily influenced …

[HTML][HTML] CRISPR/Cas9-induced knockout of miR-24 reduces cholesterol and monounsaturated fatty acid content in primary goat mammary epithelial cells

L Huang, J Luo, W Gao, N Song, H Tian, L Zhu, Q Jiang… - Foods, 2022 - mdpi.com
In nonruminants, microRNA (miRNA)-24 plays an important role in lipid metabolism in
adipose tissue and the liver. Although the abundance of miR-24 in ruminant mammary …

Identification, purification, and pharmacological activity analysis of Desmodus rotundus salivary plasminogen activator alpha1 (DSPAα1) expressed in transgenic …

T Zhang, M Zhou, H Cai, K Yan, Y Zha, W Zhuang… - Transgenic …, 2022 - Springer
Desmodus rotundus plasminogen activator alpha 1 (DSPAα1) is a thrombolytic protein with
advantages, such as a long half-life, high accuracy and specificity for thrombolysis, wide …

[HTML][HTML] Production of Bioactive Recombinant Reteplase by Virus-Based Transient Expression System in Nicotiana benthamiana

T Ma, Z Li, S Wang - Frontiers in Plant Science, 2019 - frontiersin.org
To explore a cost-effective alternative method to produce the recombinant thrombolytic drug
Reteplase (rPA), a plant viral amplicon-based gene expression system was employed to …

[HTML][HTML] Rapid Identification of HSA Genetically Modified Goats by Combining Recombinase Polymerase Amplification (RPA) with Lateral Flow Dipstick (LFD)

Q Su, K Guan, X Zhou, Y Zhou, B Liu - Agriculture, 2022 - mdpi.com
Genetically modified (GM) animals have attracted considerable attention and faced
challenges. Human serum albumin (HSA) GM goats have been used to produce goat milk …

[图书][B] Tomorrow's Healthcare by Nano-sized Approaches: A Bold Future for Medicine

P Messina, B Luciano, D Placente - 2020 - taylorfrancis.com
Nanomedicine, a scientific branch of nanotechnology that operates on the same scale as
biology, offers the possibility of influencing the healing process from inside of the body by …

Transgenesis and genetically engineered livestock as live bioreactors

B Singh, G Mal, SK Gautam, M Mukesh, B Singh… - Advances in Animal …, 2019 - Springer
Transgenic animals are important components of modern biomedical and veterinary
sciences. They produce recombinant proteins, enzymes and antibodies for treating diseases …