The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology

TE Sandberg, MJ Salazar, LL Weng, BO Palsson… - Metabolic …, 2019 - Elsevier
Harnessing the process of natural selection to obtain and understand new microbial
phenotypes has become increasingly possible due to advances in culturing techniques …

[HTML][HTML] Review of second generation bioethanol production from residual biomass

K Robak, M Balcerek - Food technology and biotechnology, 2018 - ncbi.nlm.nih.gov
In the context of climate change and the depletion of fossil fuels, there is a great need for
alternatives to petroleum in the transport sector. This review provides an overview of the …

Engineering co-utilization of glucose and xylose for chemical overproduction from lignocellulose

J Gao, W Yu, Y Li, M Jin, L Yao, YJ Zhou - Nature Chemical Biology, 2023 - nature.com
Bio-refining lignocellulose could provide a sustainable supply of fuels and fine chemicals;
however, the challenges associated with the co-utilization of xylose and glucose typically …

Big data mining, rational modification, and ancestral sequence reconstruction inferred multiple xylose isomerases for biorefinery

S Chen, Z Xu, B Ding, Y Zhang, S Liu, C Cai, M Li… - Science …, 2023 - science.org
The isomerization of xylose to xylulose is considered the most promising approach to initiate
xylose bioconversion. Here, phylogeny-guided big data mining, rational modification, and …

[HTML][HTML] Adaptive laboratory evolution–principles and applications for biotechnology

M Dragosits, D Mattanovich - Microbial cell factories, 2013 - Springer
Adaptive laboratory evolution is a frequent method in biological studies to gain insights into
the basic mechanisms of molecular evolution and adaptive changes that accumulate in …

Process design and economics for the conversion of lignocellulosic biomass to hydrocarbons: dilute-acid and enzymatic deconstruction of biomass to sugars and …

R Davis, L Tao, ECD Tan, MJ Biddy, GT Beckham… - 2013 - osti.gov
This report describes one potential conversion process to hydrocarbon products by way of
biological conversion of lingnocellulosic-dervied sugars. The process design converts …

[HTML][HTML] Selection of chromosomal DNA libraries using a multiplex CRISPR system

OW Ryan, JM Skerker, MJ Maurer, X Li, JC Tsai… - elife, 2014 - elifesciences.org
The directed evolution of biomolecules to improve or change their activity is central to many
engineering and synthetic biology efforts. However, selecting improved variants from gene …

[HTML][HTML] Xylose Fermentation by Saccharomyces cerevisiae: Challenges and Prospects

DN Moysés, VCB Reis, JRM Almeida… - International Journal of …, 2016 - mdpi.com
Many years have passed since the first genetically modified Saccharomyces cerevisiae
strains capable of fermenting xylose were obtained with the promise of an environmentally …

Microfluidic high-throughput culturing of single cells for selection based on extracellular metabolite production or consumption

BL Wang, A Ghaderi, H Zhou, J Agresti, DA Weitz… - Nature …, 2014 - nature.com
Phenotyping single cells based on the products they secrete or consume is a key bottleneck
in many biotechnology applications, such as combinatorial metabolic engineering for the …

[HTML][HTML] Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective

S Kwak, YS Jin - Microbial cell factories, 2017 - Springer
Efficient xylose utilization is one of the most important pre-requisites for developing an
economic microbial conversion process of terrestrial lignocellulosic biomass into biofuels …