Recent developments in bioprocessing of recombinant proteins: expression hosts and process development

NK Tripathi, A Shrivastava - Frontiers in Bioengineering and …, 2019 - frontiersin.org
Infectious diseases, along with cancers, are among the main causes of death among
humans worldwide. The production of therapeutic proteins for treating diseases at large …

[HTML][HTML] Metabolic engineering of Pichia pastoris

DA Peña, B Gasser, J Zanghellini, MG Steiger… - Metabolic …, 2018 - Elsevier
Besides its use for efficient production of recombinant proteins the methylotrophic yeast
Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to …

Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris

P Cai, X Wu, J Deng, L Gao, Y Shen… - Proceedings of the …, 2022 - National Acad Sciences
Methanol-based biorefinery is a promising strategy to achieve carbon neutrality goals by
linking CO2 capture and solar energy storage. As a typical methylotroph, Pichia pastoris …

Advances in the synthesis of three typical tetraterpenoids including β-carotene, lycopene and astaxanthin

Y Jing, Y Wang, D Zhou, J Wang, J Li, J Sun… - Biotechnology …, 2022 - Elsevier
Carotenoids are natural pigments that widely exist in nature. Due to their excellent
antioxidant, anticancer and anti-inflammatory properties, carotenoids are commonly used in …

[HTML][HTML] Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

J Gao, L Jiang, J Lian - Synthetic and systems biotechnology, 2021 - Elsevier
The methylotrophic yeast Pichia pastoris (aka Komagataella phaffii) is one of the most
commonly used hosts for industrial production of recombinant proteins. As a non …

Standardization of Synthetic Biology Tools and Assembly Methods for Saccharomyces cerevisiae and Emerging Yeast Species

K Malcı, E Watts, TM Roberts, JY Auxillos… - ACS synthetic …, 2022 - ACS Publications
As redesigning organisms using engineering principles is one of the purposes of synthetic
biology (SynBio), the standardization of experimental methods and DNA parts is becoming …

Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9

J Gao, J Xu, Y Zuo, C Ye, L Jiang, L Feng… - ACS Synthetic …, 2022 - ACS Publications
Pichia pastoris, an important methylotrophic yeast, is currently mainly used for the
expression of recombinant proteins and has great potential applications in the production of …

Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale

J Meng, S Liu, L Gao, K Hong, S Liu, X Wu - Microbial Cell Factories, 2023 - Springer
Background Methanol, synthesized from CO2, is a potentially sustainable one-carbon (C1)
resource for biomanufacturing. The use of methanol as a feedstock to produce single cell …

CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

Q Liu, X Shi, L Song, H Liu, X Zhou, Q Wang… - Microbial cell …, 2019 - Springer
Abstract Background Pichia pastoris (syn. Komagataella phaffii) is a widely used generally
recognized as safe host for heterologous expression of proteins in both industry and …

Current advances of Pichia pastoris as cell factories for production of recombinant proteins

Y Pan, J Yang, J Wu, L Yang, H Fang - Frontiers in Microbiology, 2022 - frontiersin.org
Pichia pastoris (syn. Komagataella spp.) has attracted extensive attention as an efficient
platform for recombinant protein (RP) production. For obtaining a higher protein titer, many …