Reverse Engineering of an Aspirin-Responsive Transcriptional Regulator in Escherichia coli

LMO Monteiro, LM Arruda… - ACS Synthetic …, 2019 - ACS Publications
Bacterial transcription factors (TFs) are key devices for the engineering of complex circuits in
many biotechnological applications, yet there are few well-characterized inducer-responsive …

Effect of Crc and Hfq proteins on the transcription, processing, and stability of the Pseudomonas putida CrcZ sRNA

S Hernández-Arranz, D Sánchez-Hevia, F Rojo… - Rna, 2016 - rnajournal.cshlp.org
In Pseudomonas putida, the Hfq and Crc proteins regulate the expression of many genes in
response to nutritional and environmental cues, by binding to mRNAs that bear specific …

[HTML][HTML] Microbial production of cis, cis-muconic acid from aromatic compounds in engineered Pseudomonas

S He, W Wang, W Wang, H Hu, P Xu, H Tang - Synthetic and Systems …, 2023 - Elsevier
Industrial expansion has led to environmental pollution by xenobiotic compounds like
polycyclic aromatic hydrocarbons and monoaromatic hydrocarbons. Pseudomonas spp …

Optimal bioprocess design through a gene regulatory network–Growth kinetic hybrid model: Towards replacing Monod kinetics

A Tsipa, M Koutinas, C Usaku, A Mantalaris - Metabolic engineering, 2018 - Elsevier
Currently, design and optimisation of biotechnological bioprocesses is performed either
through exhaustive experimentation and/or with the use of empirical, unstructured growth …

[HTML][HTML] Isolation of flurbiprofen-degrading bacteria and evaluating its degradation characteristics

T Liu, S Yang, Z Wu, Y Cai, J Zhou, M Liao - Environmental Technology & …, 2023 - Elsevier
We isolated a flurbiprofen-degrading strain LY. 1 from the water samples of the Xunsi River
in Wuhan City, China. The 16S rDNA sequence alignment showed that the LY. 1 strain was …

Preliminary study on the effect of catabolite repression gene knockout on p-nitrophenol degradation in Pseudomonas putida DLL-E4

S Li, Y Tang, L Tang, X Yan, J Xiao, H Xiang, Q Wu… - Plos one, 2022 - journals.plos.org
P-nitrophenol (PNP) is a carcinogenic, teratogenic, and mutagenic compound that can
cause serious harm to the environment. A strain of Pseudomonas putida DLL-E4, can …

Computational prediction of the Crc regulon identifies genus-wide and species-specific targets of catabolite repression control in Pseudomonas bacteria

P Browne, M Barret, F O'Gara, JP Morrissey - BMC microbiology, 2010 - Springer
Background Catabolite repression control (CRC) is an important global control system in
Pseudomonas that fine tunes metabolism in order optimise growth and metabolism in a …

Role of Acinetobacter baylyi Crc in Catabolite Repression of Enzymes for Aromatic Compound Catabolism

T Zimmermann, T Sorg, SY Siehler… - Journal of …, 2009 - Am Soc Microbiol
Here, we describe for the first time the Crc (c atabolite r epression c ontrol) protein from the
soil bacterium Acinetobacter baylyi. Expression of A. baylyi crc varied according to the …

Inactivation of Pseudomonas putida KT2440 pyruvate dehydrogenase relieves catabolite repression and improves the usefulness of this strain for degrading aromatic …

R Moreno, L Yuste, G Morales… - Microbial biotechnology, 2024 - Wiley Online Library
Pyruvate dehydrogenase (PDH) catalyses the irreversible decarboxylation of pyruvate to
acetyl‐CoA, which feeds the tricarboxylic acid cycle. We investigated how the loss of PDH …

Broadening the signal specificity of prokaryotic promoters by modifying cis-regulatory elements associated with a single transcription factor

R Silva-Rocha, V de Lorenzo - Molecular BioSystems, 2012 - pubs.rsc.org
In this report, we experimentally demonstrate that improving the cis-regulatory region of a
target promoter can significantly enhance the response to an otherwise poor inducer. The …