An Efficient Expression and Purification Protocol for SpCas9 Nuclease and Evaluation of Different Delivery Methods of Ribonucleoprotein

K Evmenov, N Pustogarov, D Panteleev… - International Journal of …, 2024 - mdpi.com
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 system is
a revolutionary tool for precise genome editing across various cell types …