Harnessing the process of natural selection to obtain and understand new microbial
phenotypes has become increasingly possible due to advances in culturing techniques …

Genome-scale stoichiometric modeling of metabolism has become a standard systems
biology tool for modeling cellular physiology and growth. Extensions of this approach are …

Metabolic engineering is the science of rewiring the metabolism of cells to enhance
production of native metabolites or to endow cells with the ability to produce new products …

Constraint-based reconstruction and analysis (COBRA) methods at the genome scale have
been under development since the first whole-genome sequences appeared in the mid …

The prediction of cellular function from a genotype is a fundamental goal in biology. For
metabolism, constraint-based modelling methods systematize biochemical, genetic and …

Adaptive laboratory evolution is a frequent method in biological studies to gain insights into
the basic mechanisms of molecular evolution and adaptive changes that accumulate in …

Experimental evolution is the study of evolutionary processes occurring in experimental
populations in response to conditions imposed by the experimenter. This research approach …

Absolute metabolite concentrations are critical to a quantitative understanding of cellular
metabolism, as concentrations impact both the free energies and rates of metabolic …

A key aim of postgenomic biomedical research is to systematically catalogue all molecules
and their interactions within a living cell. There is a clear need to understand how these …

Praise for the first edition:… superb, beautifully written and organized work that takes an
engineering approach to systems biology. Alon provides nicely written appendices to …