Different from other aerobic microorganisms that oxidise carbon sources to water and
carbon dioxide, Gluconobacter catalyses the incomplete oxidation of various substrates with …

Direct production of 2-keto-L-gulonic acid (2-KLG, the precursor of vitamin C) from D-
glucose through 2, 5-diketo-D-gluconic acid (2, 5-DKG) is a promising alternative route. To …

Gluconobacter is a potential strain for single-step production of 2-keto-L-gulonic acid (2-
KLG), which is the direct precursor of vitamin C. Three dehydrogenases, namely, sorbitol …

D-Glucose directly generates 2-keto-L-gulonic acid (2-KLG, precursor of vitamin C) through
the 2, 5-diketo-D-gluconic acid (2, 5-DKG) pathway. 2, 5-DKG is the main rate-limiting factor …

Gluconobacter oxydans is an important Gram-negative industrial microorganism that
produces vitamin C and other products due to its efficient membrane-bound dehydrogenase …

随着后基因组时代的到来, 简单高效的基因编辑工具和方法在研究微生物的基因表达调控,
工程菌株的遗传改造等方面发挥了巨大作用. 反选标记法是基于同源重组原理进行基因敲除的一 …

停滞棒杆菌是用于5! 肌苷酸生产的重要微生物底盘. 为利用代谢工程技术来创建和优化高效细胞
工厂, 在停滞棒杆菌中构建遗传改造工具和启动子文库十分必要. 本研究使用pCG1 …

L-Ascorbic acid has important roles in food (Cendrowski et al., 2021), medicine (Pardo-
Planas et al., 2017), and health (Anitra and Silvia, 2017; Reddy et al., 2020). Recent …

With the coming of the post genome era, simple and efficient gene editing tools and methods
have played a critical role in studying the regulation of gene expression and the genetic …