Towards population-scale long-read sequencing

W De Coster, MH Weissensteiner… - Nature Reviews …, 2021 - nature.com
Long-read sequencing technologies have now reached a level of accuracy and yield that
allows their application to variant detection at a scale of tens to thousands of samples …

Current status of structural variation studies in plants

Y Yuan, PE Bayer, J Batley… - Plant Biotechnology …, 2021 - Wiley Online Library
Structural variations (SVs) including gene presence/absence variations and copy number
variations are a common feature of genomes in plants and, together with single nucleotide …

Plant genome sequence assembly in the era of long reads: Progress, challenges and future directions

B Pucker, I Irisarri, J de Vries, B Xu - Quantitative Plant Biology, 2022 - cambridge.org
Third-generation long-read sequencing is transforming plant genomics. Oxford Nanopore
Technologies and Pacific Biosciences are offering competing long-read sequencing …

Low-input high-molecular-weight DNA extraction for long-read sequencing from plants of diverse families

A Russo, B Mayjonade, D Frei, G Potente… - Frontiers in Plant …, 2022 - frontiersin.org
Long-read DNA sequencing technologies require high molecular weight (HMW) DNA of
adequate purity and integrity, which can be difficult to isolate from plant material. Plant …

Exploring the gene pool of Brassica napus by genomics‐based approaches

D Hu, J Jing, RJ Snowdon, AS Mason… - Plant Biotechnology …, 2021 - Wiley Online Library
De novo allopolyploidization in Brassica provides a very successful model for reconstructing
polyploid genomes using progenitor species and relatives to broaden crop gene pools and …

Overcoming genetic paucity of Camelina sativa: possibilities for interspecific hybridization conditioned by the genus evolution pathway

RY Blume, R Kalendar, L Guo, EB Cahoon… - Frontiers in plant …, 2023 - frontiersin.org
Camelina or false flax (Camelina sativa) is an emerging oilseed crop and a feedstock for
biofuel production. This species is believed to originate from Western Asian and Eastern …

Combined use of Oxford Nanopore and Illumina sequencing yields insights into soybean structural variation biology

MA Lemay, JA Sibbesen, D Torkamaneh, J Hamel… - BMC biology, 2022 - Springer
Abstract Background Structural variants (SVs), including deletions, insertions, duplications,
and inversions, are relatively long genomic variations implicated in a diverse range of …

Local Duplication of TIR-NBS-LRR Gene Marks Clubroot Resistance in Brassica napus cv. Tosca

PM Kopec, K Mikolajczyk, E Jajor, A Perek… - Frontiers in Plant …, 2021 - frontiersin.org
Clubroot, caused by Plasmodiophora brassicae infection, is a disease of growing
importance in cruciferous crops, including oilseed rape (Brassica napus). The affected …

A novel deletion in FLOWERING LOCUS T modulates flowering time in winter oilseed rape

P Vollrath, HS Chawla, SV Schiessl, I Gabur… - Theoretical and applied …, 2021 - Springer
Key message A novel structural variant was discovered in the FLOWERING LOCUS T
orthologue BnaFT. A02 by long-read sequencing. Nested association mapping in an elite …

Accurate prediction of quantitative traits with failed SNP calls in canola and maize

SE Weber, HS Chawla, L Ehrig, LT Hickey… - Frontiers in Plant …, 2023 - frontiersin.org
In modern plant breeding, genomic selection is becoming the gold standard to select
superior genotypes in large breeding populations that are only partially phenotyped. Many …