Cryostorage management of reproductive cells and tissues in ART: status, needs, opportunities and potential new challenges

S Canosa, R Maggiulli, D Cimadomo… - Reproductive …, 2023 - Elsevier
Among the wide range of procedures performed by clinical embryologists, the
cryopreservation of reproductive cells and tissues represents a fundamental task in the daily …

The near future of vitrification of oocytes and embryos: looking into past experience and planning into the future

A Arav, Y Natan - Transfusion Medicine and Hemotherapy, 2019 - karger.com
Currently vitrification is the method of choice for low-temperature preservation of oocytes
and embryos. However, that was not the case until about 10 years ago when freezing …

Semi-automated versus manual embryo vitrification: inter-operator variability, time-saving, and clinical outcomes

N Gatimel, J Moreau, C Bettiol, J Parinaud… - Journal of Assisted …, 2021 - Springer
Purpose Does semi-automated vitrification have lower inter-operator variability and better
clinical outcomes than manual vitrification? Methods Retrospective analyses of 282 patients …

Low cryoprotectant concentration rapid vitrification of mouse oocytes and embryos

J Liu, GY Lee, JD Biggers, TL Toth, M Toner - Cryobiology, 2021 - Elsevier
Vitrification of mammalian oocytes and embryos is typically a two-step procedure involving
two solutions of increasing concentrations of cryoprotectants. In the present study, we report …

Important parameters affecting quality of vitrified donor oocytes

O Teplá, Z Topurko, J Mašata, S Jirsová, M Frolíková… - Cryobiology, 2021 - Elsevier
For group of 281 oocytes obtained from 43 stimulated donors and cryopreserved by
vitrification protocol using Cryotop and Kitazato medium we determined important …

Lack of reproducibility in oocyte vitrification calls for a simpler (whether semi-manual or automatic) and standardized methodology

A Arav, P Patrizio - Journal of assisted reproduction and genetics, 2018 - Springer
The article of Tannus et al. 2018 [1] in this issue of the journal brings to the forefront an
important issue in oocyte vitrification: the lack of reproducible results. The case reported is …

Automating Vitrification

A Arav, P Patrizio - … in Assisted Reproduction: A Practitioner's Guide to …, 2024 - Springer
Vitrification is a physical process whereby liquid is rapidly transformed into solid, amorphous
glass, thus avoiding the risk of ice crystal formation responsible for cellular death. Like any …

Improving Oocyte Freezing for Advanced Maternal Age

A Chatziparasidou - Journal of Reproductive Medicine and …, 2024 - journals.ekb.eg
Recent advancements in reproductive medicine, specifically in cryobiology and oocyte
freezing, have opened new possibilities for women seeking fertility preservation to overcome …

Development of an automated microfluidic system for the loading and unloading of cryoprotectants from mammalian oocytes

RM Zonis - 2020 - dspace.mit.edu
Oocyte cryopreservation is an assistive reproductive procedure that has allowed women to
overcome infertility and enjoy increased reproductive freedom. Despite a rapid increase in …

Automated vitrification of embryos: where do we stand?

G Nicolas, M Jessika, B Célia, P Jean… - Médecine de la …, 2019 - jle.com
La vitrification a supplanté la congélation lente pour cryopréserver les embryons humains.
Pour autant, la diversité des kits commerciaux et son caractère entièrement manuel font que …