Creative biological lignin conversion routes toward lignin valorization

ZH Liu, BZ Li, JS Yuan, YJ Yuan - Trends in Biotechnology, 2022 - cell.com
Lignin, the largest renewable aromatic resource, is a promising alternative feedstock for the
sustainable production of various chemicals, fuels, and materials. Despite this potential …

Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways

NL Bitzenhofer, L Kruse, S Thies… - Essays in …, 2021 - portlandpress.com
Biotechnological production in bacteria enables access to numerous valuable chemical
compounds. Nowadays, advanced molecular genetic toolsets, enzyme engineering as well …

Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate

E Kuatsjah, CW Johnson, D Salvachúa, AZ Werner… - Metabolic …, 2022 - Elsevier
Abstract The transformation of 4-hydroxybenzoate (4-HBA) to protocatechuate (PCA) is
catalyzed by flavoprotein oxygenases known as para-hydroxybenzoate-3-hydroxylases …

Customized valorization of waste streams by Pseudomonas putida: State-of-the-art, challenges, and future trends

J Son, SH Lim, YJ Kimm, HJ Lim, JY Lee, S Jeong… - Bioresource …, 2023 - Elsevier
Preventing catastrophic climate events warrants prompt action to delay global warming,
which threatens health and food security. In this context, waste management using …

[HTML][HTML] Paving the way for synthetic C1-metabolism in Pseudomonas putida through the reductive glycine pathway

L Bruinsma, S Wenk, NJ Claassens… - Metabolic …, 2023 - Elsevier
Abstract One-carbon (C1) compounds such as methanol, formate, and CO 2 are alternative,
sustainable microbial feedstocks for the biobased production of chemicals and fuels. In this …

A framework for the systematic selection of biosensor chassis for environmental synthetic biology

S Sridhar, CM Ajo-Franklin, CA Masiello - ACS Synthetic Biology, 2022 - ACS Publications
Microbial biosensors sense and report exposures to stimuli, thereby facilitating our
understanding of environmental processes. Successful design and deployment of …

Mercury bioremediation by engineered Pseudomonas putida KT2440 with adaptationally optimized biosecurity circuit

Y Xue, T Qiu, Z Sun, F Liu, B Yu - Environmental Microbiology, 2022 - Wiley Online Library
Hazardous materials, such as heavy metals, are the major sources of health risk. Using
genetically modified organisms (GMOs) to dispose heavy metals has the advantages of …

[HTML][HTML] Metabolic engineering of Pseudomonas putida KT2440 for medium-chain-length fatty alcohol and ester production from fatty acids

C Lu, EO Akwafo, RH Wijffels, VAPM Dos Santos… - Metabolic …, 2023 - Elsevier
Medium-chain-length fatty alcohols have broad applications in the surfactant, lubricant, and
cosmetic industries. Their acetate esters are widely used as flavoring and fragrance …

[HTML][HTML] Phosphite synthetic auxotrophy as an effective biocontainment strategy for the industrial chassis Pseudomonas putida

E Asin-Garcia, C Batianis, Y Li… - Microbial Cell …, 2022 - microbialcellfactories.biomedcentral …
The inclusion of biosafety strategies into strain engineering pipelines is crucial for safe-by-
design biobased processes. This in turn might enable a more rapid regulatory acceptance of …

SEVAtile: a standardised DNA assembly method optimised for Pseudomonas

EM Lammens, M Boon, D Grimon… - Microbial …, 2022 - Wiley Online Library
To meet the needs of synthetic biologists, DNA assembly methods have transformed from
simple 'cut‐and‐paste'procedures to highly advanced, standardised assembly techniques …