[HTML][HTML] CRISPR/Cas9-mediated genome editing techniques and new breeding strategies in cereals–current status, improvements, and perspectives

S Ahmar, G Hensel, D Gruszka - Biotechnology Advances, 2023 - Elsevier
Cereal crops, including triticeae species (barley, wheat, rye), as well as edible cereals
(wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption …

[HTML][HTML] Genome editing in cereal crops: an overview

JM Matres, J Hilscher, A Datta, V Armario-Nájera… - Transgenic …, 2021 - Springer
Genome-editing technologies offer unprecedented opportunities for crop improvement with
superior precision and speed. This review presents an analysis of the current state of …

[HTML][HTML] Genome-edited crops for improved food security of smallholder farmers

KV Pixley, JB Falck-Zepeda, RL Paarlberg… - Nature Genetics, 2022 - nature.com
Widespread enthusiasm about potential contributions of genome-edited crops to address
climate change, food security, nutrition and health, environmental sustainability and …

Superior field performance of waxy corn engineered using CRISPR–Cas9

H Gao, MJ Gadlage, HR Lafitte, B Lenderts… - Nature …, 2020 - nature.com
We created waxy corn hybrids by CRISPR–Cas9 editing of a waxy allele in 12 elite inbred
maize lines, a process that was more than a year faster than conventional trait introgression …

[HTML][HTML] Miniature type VF CRISPR-Cas nucleases enable targeted DNA modification in cells

G Bigelyte, JK Young, T Karvelis, K Budre… - Nature …, 2021 - nature.com
Class 2 CRISPR systems are exceptionally diverse, nevertheless, all share a single effector
protein that contains a conserved RuvC-like nuclease domain. Interestingly, the size of these …

[HTML][HTML] A catalogue of biochemically diverse CRISPR-Cas9 orthologs

G Gasiunas, JK Young, T Karvelis… - Nature …, 2020 - nature.com
Bacterial Cas9 nucleases from type II CRISPR-Cas antiviral defence systems have been
repurposed as genome editing tools. Although these proteins are found in many microbes …

Occurrence and nature of off-target modifications by CRISPR-Cas genome editing in plants

MHJ Sturme, JP van der Berg… - … agricultural science & …, 2022 - ACS Publications
CRISPR-Cas-based genome editing allows for precise and targeted genetic modification of
plants. Nevertheless, unintended off-target edits can arise that might confer risks when …

Plant genome editing and the relevance of off-target changes

N Graham, GB Patil, DM Bubeck, RC Dobert… - Plant …, 2020 - academic.oup.com
Site-directed nucleases (SDNs) used for targeted genome editing are powerful new tools to
introduce precise genetic changes into plants. Like traditional approaches, such as …

[HTML][HTML] Enabling trade in gene-edited produce in Asia and Australasia: The developing regulatory landscape and future perspectives

MGK Jones, J Fosu-Nyarko, S Iqbal, M Adeel… - Plants, 2022 - mdpi.com
Genome-or gene-editing (abbreviated here as 'GEd') presents great opportunities for crop
improvement. This is especially so for the countries in the Asia-Pacific region, which is home …

[HTML][HTML] Generation and molecular characterization of CRISPR/Cas9-induced mutations in 63 immunity-associated genes in tomato reveals specificity and a range of …

N Zhang, HM Roberts, J Van Eck… - Frontiers in plant …, 2020 - frontiersin.org
The CRISPR/Cas9 system is a powerful tool for targeted gene editing in many organisms
including plants. However, most of the reported uses of CRISPR/Cas9 in plants have …