[HTML][HTML] CRISPR/Cas9-mediated genome editing techniques and new breeding strategies in cereals–current status, improvements, and perspectives
Cereal crops, including triticeae species (barley, wheat, rye), as well as edible cereals
(wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption …
(wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption …
[HTML][HTML] Genome editing in cereal crops: an overview
JM Matres, J Hilscher, A Datta, V Armario-Nájera… - Transgenic …, 2021 - Springer
Genome-editing technologies offer unprecedented opportunities for crop improvement with
superior precision and speed. This review presents an analysis of the current state of …
superior precision and speed. This review presents an analysis of the current state of …
[HTML][HTML] Genome-edited crops for improved food security of smallholder farmers
KV Pixley, JB Falck-Zepeda, RL Paarlberg… - Nature Genetics, 2022 - nature.com
Widespread enthusiasm about potential contributions of genome-edited crops to address
climate change, food security, nutrition and health, environmental sustainability and …
climate change, food security, nutrition and health, environmental sustainability and …
Superior field performance of waxy corn engineered using CRISPR–Cas9
H Gao, MJ Gadlage, HR Lafitte, B Lenderts… - Nature …, 2020 - nature.com
We created waxy corn hybrids by CRISPR–Cas9 editing of a waxy allele in 12 elite inbred
maize lines, a process that was more than a year faster than conventional trait introgression …
maize lines, a process that was more than a year faster than conventional trait introgression …
[HTML][HTML] Miniature type VF CRISPR-Cas nucleases enable targeted DNA modification in cells
G Bigelyte, JK Young, T Karvelis, K Budre… - Nature …, 2021 - nature.com
Class 2 CRISPR systems are exceptionally diverse, nevertheless, all share a single effector
protein that contains a conserved RuvC-like nuclease domain. Interestingly, the size of these …
protein that contains a conserved RuvC-like nuclease domain. Interestingly, the size of these …
[HTML][HTML] A catalogue of biochemically diverse CRISPR-Cas9 orthologs
G Gasiunas, JK Young, T Karvelis… - Nature …, 2020 - nature.com
Bacterial Cas9 nucleases from type II CRISPR-Cas antiviral defence systems have been
repurposed as genome editing tools. Although these proteins are found in many microbes …
repurposed as genome editing tools. Although these proteins are found in many microbes …
Occurrence and nature of off-target modifications by CRISPR-Cas genome editing in plants
MHJ Sturme, JP van der Berg… - … agricultural science & …, 2022 - ACS Publications
CRISPR-Cas-based genome editing allows for precise and targeted genetic modification of
plants. Nevertheless, unintended off-target edits can arise that might confer risks when …
plants. Nevertheless, unintended off-target edits can arise that might confer risks when …
Plant genome editing and the relevance of off-target changes
Site-directed nucleases (SDNs) used for targeted genome editing are powerful new tools to
introduce precise genetic changes into plants. Like traditional approaches, such as …
introduce precise genetic changes into plants. Like traditional approaches, such as …
[HTML][HTML] Enabling trade in gene-edited produce in Asia and Australasia: The developing regulatory landscape and future perspectives
Genome-or gene-editing (abbreviated here as 'GEd') presents great opportunities for crop
improvement. This is especially so for the countries in the Asia-Pacific region, which is home …
improvement. This is especially so for the countries in the Asia-Pacific region, which is home …
[HTML][HTML] Generation and molecular characterization of CRISPR/Cas9-induced mutations in 63 immunity-associated genes in tomato reveals specificity and a range of …
The CRISPR/Cas9 system is a powerful tool for targeted gene editing in many organisms
including plants. However, most of the reported uses of CRISPR/Cas9 in plants have …
including plants. However, most of the reported uses of CRISPR/Cas9 in plants have …