The formation and maintenance of tissue integrity requires complex, coordinated activities
by thousands of genes and their encoded products. Until recently, transcript levels could …

The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …

The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …

CD8+ tissue resident memory T cells (TRM cells) are essential for immune defence against
pathogens and malignancies, and the molecular processes that lead to TRM cell formation …

DNA is naturally well suited to serve as a digital medium for in vivo molecular recording.
However, contemporary DNA-based memory devices are constrained in terms of the …

Molecular data storage is an attractive alternative for dense and durable information storage,
which is sorely needed to deal with the growing gap between information production and the …

The lineage relationships among the hundreds of cell types generated during development
are difficult to reconstruct. A recent method, GESTALT, used CRISPR–Cas9 barcode editing …

Many bacterial and archaeal organisms use clustered regularly interspaced short
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …

Reconstructing lineage relationships between cells within a tissue or organism is a long-
standing aim in biology. Traditionally, lineage tracing has been achieved through the …

The mammalian brain contains many specialized cells that develop from a thin sheet of
neuroepithelial progenitor cells. Single-cell transcriptomics revealed hundreds of …