[HTML][HTML] Automating the design-build-test-learn cycle towards next-generation bacterial cell factories

N Gurdo, DC Volke, D McCloskey, PI Nikel - New Biotechnology, 2023 - Elsevier
Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

The role of plant growth promoting rhizobacteria in strengthening plant resistance to fluoride toxicity: a review

A Singh, VK Yadav, H Gautam, L Rathod… - Frontiers in …, 2023 - frontiersin.org
A wide variety of bacteria are present in soil but in rhizospheric area, the majority of
microbes helps plant in defending diseases and facilitate nutrient uptake. These …

Genome analysis of a halophilic Virgibacillus halodenitrificans ASH15 revealed salt adaptation, plant growth promotion, and isoprenoid biosynthetic machinery

A Sharma, RN Singh, XP Song, RK Singh… - Frontiers in …, 2023 - frontiersin.org
Globally, due to widespread dispersion, intraspecific diversity, and crucial ecological
components of halophilic ecosystems, halophilic bacteria is considered one of the key …

RB-TnSeq identifies genetic targets for improved tolerance of Pseudomonas putida towards compounds relevant to lignin conversion

AJ Borchert, A Bleem, GT Beckham - Metabolic Engineering, 2023 - Elsevier
Lignin-derived mixtures intended for bioconversion commonly contain high concentrations
of aromatic acids, aliphatic acids, and salts. The inherent toxicity of these chemicals places a …

Recombinant Pseudomonas growing on non-natural fluorinated substrates shows stress but overall tolerance to cytoplasmically released fluoride anion

AG Dodge, CJ Thoma, MR O'Connor, LP Wackett - Mbio, 2024 - Am Soc Microbiol
Microbial response to toxic fluoride anion has traditionally been studied by adding inorganic
fluoride salts to growth media. Fluoride is known to spontaneously transit the membrane as …

Fluoride export is required for the competitive fitness of pathogenic microorganisms in dental biofilm models

A Banerjee, CY Kang, M An, BB Koff, S Sunder… - Mbio, 2024 - Am Soc Microbiol
Microorganisms resist fluoride toxicity using fluoride export proteins from one of several
different molecular families. Cariogenic species Streptococcus mutans and Candida …

The link between ancient microbial fluoride resistance mechanisms and bioengineering organofluorine degradation or synthesis

RB Stockbridge, LP Wackett - Nature Communications, 2024 - nature.com
Fluorinated organic chemicals, such as per-and polyfluorinated alkyl substances (PFAS)
and fluorinated pesticides, are both broadly useful and unusually long-lived. To combat …

Evolutionary obstacles and not C–F bond strength make PFAS persistent

LP Wackett - Microbial Biotechnology, 2024 - Wiley Online Library
The fate of organic matter in the environment, including anthropogenic chemicals, is largely
predicated on the enzymatic capabilities of microorganisms. Microbes readily degrade, and …

Distribution characteristics and risk assessment of fluoride in surface water of urban typical rivers

R Mu, K Cui, Y Chen, Y Tang, K Wang, S Sun - Science of The Total …, 2024 - Elsevier
Recent research on fluoride in water primarily focuses on groundwater; however, the
potential environmental risks of fluoride in urban rivers should not be overlooked. In 2023 …

Purification and characterization of catechol 1, 2-dioxygenase (EC 1.13. 11.1; catechol-oxygen 1, 2-oxidoreductase; C12O) using the local isolate of phenol-degrading …

HR Tawfeeq, SS Al-Jubori, AH Mussa - Folia Microbiologica, 2024 - Springer
The purpose of the present study was to purify and characterize the catechol 1, 2-
dioxygenase (EC 1.13. 11.1; catechol-oxygen 1, 2-oxidoreductase; C12O) enzyme from the …