The rise of the “Top Down” method in the field of mass spectrometry-based proteomics has
ushered in a new age of promise and challenge for the characterization and identification of …

Mass spectrometry (MS)–based high-throughput proteomics is the core technique for large-
scale protein characterization. Due to the extreme complexity of proteomes, sophisticated …

Introduction In proteomics a paradox situation developed in the last years. At one side it is
basic knowledge that proteins are post-translationally modified and occur in different …

Over the last years, top‐down (TD) MS has gained a remarkable space in proteomics,
rapidly trespassing the limit between a promising approach and a solid, established …

This review intends not only to discuss the current possibilities to gain 100% sequence
coverage for proteins, but also to point out the critical limits to such an attempt. The aim of …

Although cockroaches are known to produce allergens that can cause IgE‐mediated
hypersensitivity reactions, including perennial rhinitis and asthma, the various cockroach …

Protein isoforms/splice variants can play important roles in various biological processes and
can potentially be used as biomarkers or therapeutic targets/mediators. Thus, there is a …

Mass spectrometry used in combination with a wide variety of separation methods is the
principal methodology for proteomics. In bottom-up approach, proteins are cleaved with a …

Due to the complex nature of the proteome, instrumentation and methods development for
sample cleanup, fractionation, preconcentration, chromatographic separation and detection …

Despite the availability of ultra-high-resolution mass spectrometers, methods for separation
and detection of intact proteins for proteome-scale analyses are still in a developmental …