[HTML][HTML] A review on biological synthesis of the biodegradable polymers polyhydroxyalkanoates and the development of multiple applications

B Dalton, P Bhagabati, J De Micco, RB Padamati… - Catalysts, 2022 - mdpi.com
Polyhydroxyalkanoates, or PHAs, belong to a class of biopolyesters where the
biodegradable PHA polymer is accumulated by microorganisms as intracellular granules …

[HTML][HTML] Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

[HTML][HTML] Modular (de) construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing

DC Volke, RA Martino, E Kozaeva, AM Smania… - Nature …, 2022 - nature.com
CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

PI Nikel, T Fuhrer, M Chavarría… - The ISME …, 2021 - academic.oup.com
As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …

Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates

MP Mezzina, MT Manoli, MA Prieto… - Biotechnology …, 2021 - Wiley Online Library
Growing environmental concern sparks renewed interest in the sustainable production of
(bio) materials that can replace oil‐derived goods. Polyhydroxyalkanoates (PHAs) are …

Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways

NL Bitzenhofer, L Kruse, S Thies… - Essays in …, 2021 - portlandpress.com
Biotechnological production in bacteria enables access to numerous valuable chemical
compounds. Nowadays, advanced molecular genetic toolsets, enzyme engineering as well …

Exploiting unconventional prokaryotic hosts for industrial biotechnology

B Blombach, A Grünberger, F Centler, N Wierckx… - Trends in …, 2022 - cell.com
Developing cost-efficient biotechnological processes is a major challenge in replacing fossil-
based industrial production processes. The remarkable progress in genetic engineering …

[HTML][HTML] Paving the way for synthetic C1-Metabolism in Pseudomonas putida through the reductive glycine pathway

L Bruinsma, S Wenk, NJ Claassens… - Metabolic …, 2023 - Elsevier
Abstract One-carbon (C1) compounds such as methanol, formate, and CO 2 are alternative,
sustainable microbial feedstocks for the biobased production of chemicals and fuels. In this …

RB-TnSeq identifies genetic targets for improved tolerance of Pseudomonas putida towards compounds relevant to lignin conversion

AJ Borchert, A Bleem, GT Beckham - Metabolic Engineering, 2023 - Elsevier
Lignin-derived mixtures intended for bioconversion commonly contain high concentrations
of aromatic acids, aliphatic acids, and salts. The inherent toxicity of these chemicals places a …

Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate

E Kuatsjah, CW Johnson, D Salvachúa, AZ Werner… - Metabolic …, 2022 - Elsevier
Abstract The transformation of 4-hydroxybenzoate (4-HBA) to protocatechuate (PCA) is
catalyzed by flavoprotein oxygenases known as para-hydroxybenzoate-3-hydroxylases …