Highlights•Chronic stresses related to biotechnology applications are focused.•Epigenetic
regulation benefits dynamic regulation of stress tolerance.•Yeast chemical stress response …

Recent advancements in biosensor technology have revolutionized the field of microbial
engineering, enabling efficient and precise optimization of strains for the production of …

The CRISPR-Cas system has enabled the development of sophisticated, multigene
metabolic engineering programs through the use of guide RNA-directed activation or …

Improving our understanding of the transcriptional changes of Saccharomyces cerevisiae
during fermentation of lignocellulosic hydrolysates is crucial for the creation of more efficient …

With the expansion of domesticated microbes producing biomaterials and chemicals to
support a growing circular bioeconomy, the variety of waste and sustainable substrates that …

Formic acid is one of the main weak acids in lignocellulosic hydrolysates that is known to be
inhibitory to yeast growth even at low concentrations. In this study, we employed a CRISPR …

Transcription factor (TF)-based biosensors are important tools in strain development and
screening as they can allow accurate monitoring of intracellular concentrations of a …

This dissertation partially fulfils the requirements for obtaining the degree of Doctor of
Philosophy at the Department of Life Sciences at Chalmers University of Technology. The …

Bioprocesses enable the efficient production of valuable chemicals by microorganisms such
as the yeast Saccharomyces cerevisiae. Predictable and stochastic perturbations affect …

Lignocellulosic biomass, residual biomass from the agricultural and forestry industries, is the
most abundant organic substance on the planet. For efficient utilization by Saccharomyces …