Robust, 3-dimensional visualization of human colon enteric nervous system without tissue sectioning

KD Graham, SH López, R Sengupta, A Shenoy… - Gastroenterology, 2020 - Elsevier
Background & Aims Small, 2-dimensional sections routinely used for human pathology
analysis provide limited information about bowel innervation. We developed a technique to …

[HTML][HTML] Sympathetic pathways target cholinergic neurons in the human colonic myenteric plexus

DR Parker, L Wiklendt, A Humenick, BN Chen… - Frontiers in …, 2022 - frontiersin.org
Background The sympathetic nervous system inhibits human colonic motility largely by
effects on enteric neurons. Noradrenergic axons, which branch extensively in the myenteric …

Characterization of putative interneurons in the myenteric plexus of human colon

A Humenick, BN Chen, DA Wattchow… - …, 2021 - Wiley Online Library
Background The enteric nervous system contains multiple classes of neurons,
distinguishable by morphology, immunohistochemical markers, and projections; however …

Quantitative analysis of enteric neurons containing choline acetyltransferase and nitric oxide synthase immunoreactivities in the submucosal and myenteric plexuses …

M Mazzoni, F Caremoli, L Cabanillas… - Cell and tissue …, 2021 - Springer
The enteric nervous system (ENS) controls gastrointestinal functions. In large mammals'
intestine, it comprises an inner (ISP) and outer (OSP) submucous plexus and a myenteric …

Characterization of projections of longitudinal muscle motor neurons in human colon

A Humenick, BN Chen, CIW Lauder… - …, 2019 - Wiley Online Library
Background The enteric nervous system contains inhibitory and excitatory motor neurons
which modulate smooth muscle contractility. Cell bodies of longitudinal muscle motor …

[HTML][HTML] Association of serum neuron-specific enolase and C-reactive protein with disease location and endoscopic inflammation degree in patients with Crohn's …

RX Yang, WJ Song, ZQ Wu, H Goyal, HG Xu - Frontiers in Medicine, 2021 - frontiersin.org
Objective: The objective of this study was to explore the association between serum markers
neuron-specific enolase (NSE) and C-reactive protein (CRP) with intestinal lesion location …

[HTML][HTML] Automated computational analysis reveals structural changes in the enteric nervous system of nNOS deficient mice

BR Cairns, B Jevans, A Chanpong, D Moulding… - Scientific Reports, 2021 - nature.com
Neuronal nitric oxide synthase (nNOS) neurons play a fundamental role in inhibitory
neurotransmission, within the enteric nervous system (ENS), and in the establishment of gut …

[HTML][HTML] Antibody elution with 2-me/SDS solution: Uses for multi-layer immunohistochemical analysis of wholemount preparations of human colonic myenteric plexus

A Humenick, ME Johnson, BN Chen, M Wee… - Heliyon, 2024 - cell.com
Indirect immunofluorescence is usually restricted to 3-5 markers per preparation, limiting
analysis of coexistence. A solution containing 2-mercaptoethanol and sodium dodecyl …

New insights into the characterization of the mechanism of action of hyoscine butylbromide in the human colon ex vivo

S Traserra, LG Alcalá-González, C Barber… - European Journal of …, 2024 - Elsevier
Introduction Hyoscine butylbromide (HBB) is one of the most used antispasmodics in clinical
practice. Recent translational consensus has demonstrated a similarity between human …

[HTML][HTML] Types of neurons in the human colonic myenteric plexus identified by multilayer immunohistochemical coding

BN Chen, A Humenick, WP Yew, RA Peterson… - Cellular and Molecular …, 2023 - Elsevier
Background and Aims Gut functions including motility, secretion, and blood flow are largely
controlled by the enteric nervous system. Characterizing the different classes of enteric …