Characterizing extracellular vesicles and their diverse RNA contents

EM Veziroglu, GI Mias - Frontiers in Genetics, 2020 - frontiersin.org
Cells release nanometer-scale, lipid bilayer-enclosed biomolecular packages (extracellular
vesicles; EVs) into their surrounding environment. EVs are hypothesized to be intercellular …

Viscoelastic microfluidics: Progress and challenges

J Zhou, I Papautsky - Microsystems & Nanoengineering, 2020 - nature.com
The manipulation of cells and particles suspended in viscoelastic fluids in microchannels
has drawn increasing attention, in part due to the ability for single-stream three-dimensional …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

Label-free microfluidic cell sorting and detection for rapid blood analysis

N Lu, HM Tay, C Petchakup, L He, L Gong, KK Maw… - Lab on a Chip, 2023 - pubs.rsc.org
Blood tests are considered as standard clinical procedures to screen for markers of diseases
and health conditions. However, the complex cellular background (> 99.9% RBCs) and …

Guidelines for the use of flow cytometry and cell sorting in immunological studies*

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2017 - Wiley Online Library
From a technical point of view a flow cytometer is a light detection device capable of
detecting photons of different wavelengths over a high dynamic range. In order to achieve a …

Towards microfluidic-based exosome isolation and detection for tumor therapy

J Wang, P Ma, DH Kim, BF Liu, U Demirci - Nano Today, 2021 - Elsevier
Exosomes are a class of cell-secreted, nano-sized extracellular vesicles with a bilayer
membrane structure of 30–150 nm in diameter. Their discovery and application have …

Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine

JC Contreras-Naranjo, HJ Wu, VM Ugaz - Lab on a Chip, 2017 - pubs.rsc.org
Exosomes, the smallest sized extracellular vesicles (∽ 30–150 nm) packaged with lipids,
proteins, functional messenger RNAs and microRNAs, and double-stranded DNA from their …

Biomedical applications of microfluidic devices: a review

G Gharib, İ Bütün, Z Muganlı, G Kozalak, İ Namlı… - Biosensors, 2022 - mdpi.com
Both passive and active microfluidic chips are used in many biomedical and chemical
applications to support fluid mixing, particle manipulations, and signal detection. Passive …

Progress in microfluidics‐based exosome separation and detection technologies for diagnostic applications

S Lin, Z Yu, D Chen, Z Wang, J Miao, Q Li, D Zhang… - Small, 2020 - Wiley Online Library
Exosomes are secreted by most cell types and circulate in body fluids. Recent studies have
revealed that exosomes play a significant role in intercellular communication and are closely …

The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits

SZ Shirejini, F Inci - Biotechnology Advances, 2022 - Elsevier
Exosomes are a subset of extracellular vesicles released from various cells, and they can be
found in different bodily fluids. Exosomes have been utilized as biomarkers to diagnose …