The application of sequencing technology is shifting from research to clinical laboratories
owing to rapid technological developments and substantially reduced costs. However …

Background Tuberculous meningitis (TBM) is a severe form of extrapulmonary tuberculosis
and its early diagnosis is very difficult leading to present with severe disability or die. The …

The unmapped readspace of whole genome sequencing data tends to be large but is often
ignored. We posit that it contains valuable signals of both human infection and …

Objectives The COVID-19 pandemic has underscored the need for rapid novel diagnostic
strategies. Metagenomic Next-Generation Sequencing (mNGS) may allow for the detection …

Background Central nervous system (CNS) infections pose a fatal risk to patients. However,
the limited sample volumes of cerebrospinal fluid (CSF) and low detection efficiency …

Next-generation sequencing (NGS) yields powerful opportunities for studying human
papillomavirus (HPV) genomics for applications in epidemiology, public health, and clinical …

Infectious diseases pose a fatal risk to neonates. Timely and accurate pathogen detection is
crucial for proper clinical diagnosis and therapeutic strategies. Limited sample volumes from …

Metagenomic next-generation sequencing (mNGS) is an emerging sequence-based method
that detects genomic information of pathogenic microorganisms from a wide range of clinical …

Objective Pneumocystis jirovecii pneumonia (PJP) can be a life-threatening opportunistic
infection. We aimed to evaluate the diagnostic accuracy of metagenomic next-generation …

Objectives Metagenomic next-generation sequencing (mNGS) has been applied more and
more widely for the diagnosis of infectious diseases, but its performance in the diagnosis of …