CRISPR/Cas detection with nanodevices: moving deeper into liquid biopsy

H Kong, K Yi, RL Mintz, B Wang, Y Xu… - Chemical …, 2024 - pubs.rsc.org
The emerging field of liquid biopsy has garnered significant interest in precision diagnostics,
offering a non-invasive and repetitive method for analyzing bodily fluids to procure real-time …

Recent advances in chemical biology tools for protein and RNA profiling of extracellular vesicles

W Lim, S Lee, M Koh, A Jo, J Park - RSC Chemical Biology, 2024 - pubs.rsc.org
Extracellular vesicles (EVs) are nano-sized vesicles secreted by cells and contain various
cellular components such as proteins, nucleic acids, and lipids from the parent cell. EVs are …

Simultaneous subset tracing and miRNA profiling of tumor-derived exosomes via dual-surface-protein orthogonal barcoding

Y Lei, X Fei, Y Ding, J Zhang, G Zhang, L Dong… - Science …, 2023 - science.org
The clinical potential of miRNA-based liquid biopsy has been largely limited by the
heterogeneous sources in plasma and tedious assay processes. Here, we develop a …

Low background self-primer EXPAR coupled with colorimetric and lateral flow assay for rapid and sensitive point-of-care detection of miRNA

J Peng, X Hang, H Wang, K Zhao, H Wang, H Yu… - Sensors and Actuators B …, 2024 - Elsevier
MicroRNAs (miRNAs) have emerged as powerful biomarkers for various diseases
diagnosis, yet their clinical application is greatly plagued by the lack of reliable and rapid …

Natural Killer Cell‐Derived Extracellular Vesicles as Potential Anti‐Viral Nanomaterials

SA Lim, N Ho, S Chen, EJ Chung - Advanced Healthcare …, 2024 - Wiley Online Library
In viral infections, natural killer (NK) cells exhibit anti‐viral activity by inducing apoptosis in
infected host cells and impeding viral replication through heightened cytokine release …

Current strategies for early epithelial ovarian cancer detection using miRNA as a potential tool

M Bhadra, M Sachan, S Nara - Frontiers in Molecular Biosciences, 2024 - frontiersin.org
Ovarian cancer is one of the most aggressive and significant malignant tumor forms in the
female reproductive system. It is the leading cause of death among gynecological cancers …

Application of CRISPR/Cas13a-based biosensors in serum marker detection

W He, X Liu, J Na, H Bian, L Zhong, G Li - Analytical Methods, 2024 - pubs.rsc.org
The detection of serum markers is important for the early diagnosis and monitoring of
diseases, but conventional detection methods have the problem of low specificity or …

Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review

Z Wang, X Zhou, Q Kong, H He, J Sun, W Qiu… - Advanced …, 2024 - Wiley Online Library
In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the
life sciences due to their critical roles in both health and disease states, offering promising …

[HTML][HTML] Advances in application of CRISPR-Cas13a system

Y Zhang, S Li, R Li, X Qiu, T Fan, B Wang… - Frontiers in Cellular …, 2024 - frontiersin.org
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) and CRISPR-
associated (Cas) proteins serve as an adaptive immune system that safeguards prokaryotes …

[HTML][HTML] Advances in CRISPR Based Biosensing Strategies for Cancer Diagnosis

QL Yang, XY Zhong, LM Zhong… - Cancer Screening …, 2024 - xiahepublishing.com
With the development of gene editing technology, its application in tumor diagnosis is
becoming increasingly widespread. The CRISPR/Cas system is an important gene editing …