Protein phosphorylation is a post-translational modification (PTM) that orchestrates a diverse
array of cellular processes. Because this modification serves as a rapid and reversible …

Quantitative measurement of proteins is one of the most fundamental analytical tasks in a
biochemistry laboratory, but widely used immunochemical methods often have limited …

Mass spectrometry has transformed the field of cell signaling by enabling global studies of
dynamic protein phosphorylation ('phosphoproteomics'). Recent developments are enabling …

Protein methylation is a common posttranslational modification that mostly occurs on
arginine and lysine residues. Arginine methylation has been reported to regulate RNA …

Eukaryotic cells replicate by a complex series of evolutionarily conserved events that are
tightly regulated at defined stages of the cell division cycle. Progression through this cycle …

N-linked glycosylation is a biologically important protein modification, but only a small
fraction of modification sites have been mapped. We developed a" filter aided sample …

The histone acetyltransferase (HAT) p300/CBP is a transcriptional coactivator implicated in
many gene regulatory pathways and protein acetylation events. Although p300 inhibitors …

SILAC is based on direct addition of selected stable isotope amino acids into the cell culture
medium, allowing superior quantitative analysis of the cellular proteome compared to other …

Signalling networks regulate essentially all of the biology of cells and organisms in normal
and disease states. Signalling is often studied using antibody-based techniques such as …

This review discusses extracellular vesicles (EVs), which are submicron-scale, anuclear,
phospholipid bilayer membrane enclosed vesicles that contain lipids, metabolites, proteins …