Precise plant genome editing using base editors and prime editors

KA Molla, S Sretenovic, KC Bansal, Y Qi - Nature Plants, 2021 - nature.com
The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …

Applications of CRISPR–Cas in agriculture and plant biotechnology

H Zhu, C Li, C Gao - Nature Reviews Molecular Cell Biology, 2020 - nature.com
The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …

PAM-less plant genome editing using a CRISPR–SpRY toolbox

Q Ren, S Sretenovic, S Liu, X Tang, L Huang, Y He… - Nature plants, 2021 - nature.com
The rapid development of the CRISPR–Cas9,–Cas12a and–Cas12b genome editing
systems has greatly fuelled basic and translational plant research,,,,–. DNA targeting by …

[HTML][HTML] The integrated genomics of crop domestication and breeding

X Huang, S Huang, B Han, J Li - Cell, 2022 - cell.com
As a major event in human civilization, wild plants were successfully domesticated to be
crops, largely owing to continuing artificial selection. Here, we summarize new discoveries …

[HTML][HTML] CRISPR/Cas: A powerful tool for gene function study and crop improvement

D Zhang, Z Zhang, T Unver, B Zhang - Journal of Advanced Research, 2021 - Elsevier
Background It is a long-standing goal of scientists and breeders to precisely control a gene
for studying its function as well as improving crop yield, quality, and tolerance to various …

Plant base editing and prime editing: the current status and future perspectives

J Li, C Zhang, Y He, S Li, L Yan, Y Li… - Journal of Integrative …, 2023 - Wiley Online Library
Precise replacement of an allele with an elite allele controlling an important agronomic trait
in a predefined manner by gene editing technologies is highly desirable in crop …

[HTML][HTML] A critical review: recent advancements in the use of CRISPR/Cas9 technology to enhance crops and alleviate global food crises

A Rasheed, RA Gill, MU Hassan, A Mahmood… - Current Issues in …, 2021 - mdpi.com
Genome editing (GE) has revolutionized the biological sciences by creating a novel
approach for manipulating the genomes of living organisms. Many tools have been …

[HTML][HTML] Development of plant prime-editing systems for precise genome editing

R Xu, J Li, X Liu, T Shan, R Qin, P Wei - Plant Communications, 2020 - cell.com
Prime-editing systems have the capability to perform efficient and precise genome editing in
human cells. In this study, we first developed a plant prime editor 2 (pPE2) system and test …

[HTML][HTML] Natural variation in crops: realized understanding, continuing promise

Y Liang, HJ Liu, J Yan, F Tian - Annual review of plant biology, 2021 - annualreviews.org
Crops feed the world's population and shape human civilization. The improvement of crop
productivity has been ongoing for almost 10,000 years and has evolved from an experience …

Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy

X Huang, H Jia, J Xu, Y Wang, J Wen, N Wang - Nature Plants, 2023 - nature.com
Transgene-free plant genome editing in the T0 generation is highly desirable but
challenging,. Here we achieved such a goal using a co-editing strategy via Agrobacterium …