Optical spectroscopy at the ultimate limit of a single molecule has grown over the past dozen
years into a powerful technique for exploring the individual nanoscale behavior of molecules …

Efficient collection and detection of fluorescence coupled with careful minimization of
background from impurities and Raman scattering now enable routine optical microscopy …

The photostability of fluorescent dyes is of crucial importance for the statistical accuracy of
single-molecule detection (SMD) and for the image quality of scanning confocal microscopy …

In recent years fluorescence correlation spectroscopy (FCS) has become a routine method
for determining diffusion coefficients, chemical rate constants, molecular concentrations …

(60) Provisional application No. 60/547,611, filed on Feb. 5,405,783 A 4/1995 Pirrung et al.
24, 2004, provisional application No. 60/546,277. 5,409,811 A 4/1995 Tabor et al. filed on …

We report the application of confocal imaging and fluorescence correlation spectroscopy
(FCS) to characterize chemically well-defined lipid bilayer models for biomembranes. Giant …

(54) METHODS AND APPARATUS FOR 4,962,037 A 10/1990 Jett et al. ANALYZING
POLYNUCLEOTIDE 4,971,903 A 11/1990 Hyman SEQUENCES BY ASYNCHRONOUS …

Abstract Knowledge of the three-dimensional structures of protein targets now emerging
from genomic data has the potential to accelerate drug discovery greatly. X-ray …

We report the synthesis of MOF@ lipid nanoparticles as a versatile and powerful novel class
of nanocarriers based on metal–organic frameworks (MOFs). We show that the MOF@ lipid …

▪ Abstract Recent developments in optical studies of single molecules at room temperature
are reviewed, with an emphasis on the underlying principles and the potential of single …