Human milk oligosaccharides are complex, indigestible oligosaccharides that provide ideal
nutrition for infant development. Here, 2′-fucosyllactose was efficiently produced in …

Fucosyllactose (2′-FL) is an important functional ingredient of advanced infant formula.
Here, Escherichia coli MG1655 was engineered for achieving high 2′-FL production. The …

Abstract Lacto-N-fucopentaose I (LNFP I) is an abundant and important fucosylated human
milk oligosaccharide (HMO). Here, an efficient LNFP I-producing strain without by-product …

2′-Fucosyllactose (2′-FL) is a kind of fucosylated human milk oligosaccharide (HMO),
representing the most abundant oligosaccharide in breast milk. We conducted systematic …

Highlights•Human milk oligosaccharides (HMOs) are widely recognized as an important
element of infant nutrition.•Recent efforts in the field of metabolic engineering have yielded …

Lacto-N-neotetraose (LNnT) is a neutral human milk oligosaccharide with important
biological functions. However, the low LNnT productivity and the incomplete conversion of …

3-Fucosyllactose (3-FL) is an important oligosaccharide and nutrient in breast milk that can
be synthesized in microbial cells by α-1, 3-fucosyltransferase (α-1, 3-FucT) using guanosine …

Fucosyllactose (FL) has garnered considerable attention for its benefits on infant health. In
this study, we report an efficient E. coli cell factory to produce 2′/3-fucosyllactose (2′/3-FL) …

3′-Sialyllactose (3′-SL) is among the foremost and simplest sialylated breast milk
oligosaccharides. In this study, an engineered Escherichia coli for high-titer 3′-SL …

Sialyllactose (6′-SL), an abundant sialylated human milk oligosaccharide, has attracted
considerable attention for its potential applications in nutritional healthcare and …