The yeast deletion collection: a decade of functional genomics
The yeast deletion collections comprise> 21,000 mutant strains that carry precise start-to-
stop deletions of∼ 6000 open reading frames. This collection includes heterozygous and …
stop deletions of∼ 6000 open reading frames. This collection includes heterozygous and …
Budding Yeast for Budding Geneticists: A Primer on the Saccharomyces cerevisiae Model System
AA Duina, ME Miller, JB Keeney - Genetics, 2014 - academic.oup.com
The budding yeast Saccharomyces cerevisiae is a powerful model organism for studying
fundamental aspects of eukaryotic cell biology. This Primer article presents a brief historical …
fundamental aspects of eukaryotic cell biology. This Primer article presents a brief historical …
A global genetic interaction network maps a wiring diagram of cellular function
INTRODUCTION Genetic interactions occur when mutations in two or more genes combine
to generate an unexpected phenotype. An extreme negative or synthetic lethal genetic …
to generate an unexpected phenotype. An extreme negative or synthetic lethal genetic …
Systematic analysis of complex genetic interactions
INTRODUCTION Genetic interactions occur when mutations in different genes combine to
result in a phenotype that is different from expectation based on those of the individual …
result in a phenotype that is different from expectation based on those of the individual …
[HTML][HTML] The inner nuclear membrane is a metabolically active territory that generates nuclear lipid droplets
A Romanauska, A Köhler - Cell, 2018 - cell.com
The inner nuclear membrane (INM) encases the genome and is fused with the outer nuclear
membrane (ONM) to form the nuclear envelope. The ONM is contiguous with the …
membrane (ONM) to form the nuclear envelope. The ONM is contiguous with the …
Precise, automated control of conditions for high-throughput growth of yeast and bacteria with eVOLVER
BG Wong, CP Mancuso, S Kiriakov, CJ Bashor… - Nature …, 2018 - nature.com
Precise control over microbial cell growth conditions could enable detection of minute
phenotypic changes, which would improve our understanding of how genotypes are shaped …
phenotypic changes, which would improve our understanding of how genotypes are shaped …
Systematic humanization of yeast genes reveals conserved functions and genetic modularity
To determine whether genes retain ancestral functions over a billion years of evolution and
to identify principles of deep evolutionary divergence, we replaced 414 essential yeast …
to identify principles of deep evolutionary divergence, we replaced 414 essential yeast …
High-content screening for quantitative cell biology
High-content screening (HCS), which combines automated fluorescence microscopy with
quantitative image analysis, allows the acquisition of unbiased multiparametric data at the …
quantitative image analysis, allows the acquisition of unbiased multiparametric data at the …
Affinity‐purification coupled to mass spectrometry: Basic principles and strategies
WH Dunham, M Mullin, AC Gingras - Proteomics, 2012 - Wiley Online Library
Identifying the interactions established by a protein of interest can be a critical step in
understanding its function. This is especially true when an unknown protein of interest is …
understanding its function. This is especially true when an unknown protein of interest is …
In‐depth and 3‐dimensional exploration of the budding yeast phosphoproteome
Phosphorylation is one of the most dynamic and widespread post‐translational
modifications regulating virtually every aspect of eukaryotic cell biology. Here, we assemble …
modifications regulating virtually every aspect of eukaryotic cell biology. Here, we assemble …