Mass spectrometry–based quantitative proteomics is a powerful tool for gaining insights into
function and dynamics of biological systems. However, peptides with different sequences …

Proteomics is a large-scale study of proteins, aiming at the description and characterization
of all expressed proteins in biological systems. The expressed proteins are typically highly …

Senescent cells are beneficial for repairing acute tissue damage, but they are harmful when
they accumulate in tissues, as occurs with advancing age. Senescence-associated …

The multiplexing capabilities of isobaric mass tag-based protein quantification, such as
Tandem Mass Tags or Isobaric Tag for Relative and Absolute Quantitation have dramatically …

Primary cilia are sensory, antennae‐like organelles present on the surface of many cell
types. They have been involved in a variety of diseases collectively termed ciliopathies. As …

MS1-based label-free quantification can compare precursor ion peaks across runs, allowing
reproducible protein measurements. Among bioinformatic platforms enabling MS1-based …

Label-free shotgun proteomics is routinely used to assess proteomes. However, extracting
relevant information from the massive amounts of generated data remains difficult. This …

Missing values are a major issue in quantitative data-dependent mass spectrometry-based
proteomics. We therefore present an innovative solution to this key issue by introducing a …

Protein glycosylation is one of the most important posttranslational modifications. Numerous
biological functions are related to protein glycosylation. However, analytical challenges …

Two-dimensional gel electrophoresis (2DE) is an important and well-established technical
platform enabling extensive top-down proteomic analysis. However, the long-held but now …