The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction
PL Wale, DK Gardner - Human reproduction update, 2016 - academic.oup.com
BACKGROUND Although laboratory procedures, along with culture media formulations,
have improved over the past two decades, the issue remains that human IVF is performed in …
have improved over the past two decades, the issue remains that human IVF is performed in …
Culture and selection of viable blastocysts: a feasible proposition for human IVF?
DK Gardner, M Lane - Human reproduction update, 1997 - academic.oup.com
In human in-vitro fertilization (IVF) embryos are routinely transferred to the uterus on day 2 or
day 3 of development. Resultant implantation and pregnancy rates are disappointingly low …
day 3 of development. Resultant implantation and pregnancy rates are disappointingly low …
A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization.
DK Gardner, WB Schoolcraft, L Wagley… - Human reproduction …, 1998 - academic.oup.com
The effectiveness of blastocyst culture and transfer in human in-vitro fertilization (IVF) was
evaluated in a prospective randomized trial in patients having a moderate to good response …
evaluated in a prospective randomized trial in patients having a moderate to good response …
[HTML][HTML] Redox Regulation and Oxidative Stress in Mammalian Oocytes and Embryos Developed In Vivo and In Vitro
Oocytes and preimplantation embryos require careful regulation of the redox environment
for optimal development both in vivo and in vitro. Reactive oxygen species (ROS) are …
for optimal development both in vivo and in vitro. Reactive oxygen species (ROS) are …
Mitochondrial distribution and adenosine triphosphate content of bovine oocytes before and after in vitro maturation: correlation with morphological criteria and …
M Stojkovic, SA Machado, P Stojkovic… - Biology of …, 2001 - academic.oup.com
In this study, we evaluated mitochondrial distribution and ATP content of individual bovine
oocytes before and after in vitro maturation (IVM). Cumulus-oocyte complexes were …
oocytes before and after in vitro maturation (IVM). Cumulus-oocyte complexes were …
The origin and efficient derivation of embryonic stem cells in the mouse
FA Brook, RL Gardner - Proceedings of the National …, 1997 - National Acad Sciences
By explanting tissues isolated microsurgically from implanting strain 129 mouse blastocysts
individually on STO feeder cells we have established that embryonic stem (ES) cells …
individually on STO feeder cells we have established that embryonic stem (ES) cells …
Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: amino acids …
DK Gardner, M Lane, A Spitzer… - Biology of reproduction, 1994 - academic.oup.com
The aim of this study was to develop a serum-free culture system that could support high
levels of cleavage and blastocyst formation from sheep zygotes developed in vitro. To this …
levels of cleavage and blastocyst formation from sheep zygotes developed in vitro. To this …
Environment of the preimplantation human embryo in vivo: metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells
DK Gardner, M Lane, I Calderon, J Leeton - Fertility and sterility, 1996 - Elsevier
Objective To determine the levels of metabolites surrounding the human oocyte and embryo
in vivo. Design Oviduct and uterine fluids were collected throughout the menstrual cycle …
in vivo. Design Oviduct and uterine fluids were collected throughout the menstrual cycle …
Changes in requirements and utilization of nutrients during mammalian preimplantation embryo development and their significance in embryo culture
DK Gardner - Theriogenology, 1998 - Elsevier
Along with the transition from maternal to embryonic genome control the mammalian
preimplantation embryo undergoes significant changes in its physiology during …
preimplantation embryo undergoes significant changes in its physiology during …
[图书][B] Textbook of assisted reproductive techniques
DK Gardner, A Weissman, CM Howles, Z Shoham - 2018 - api.taylorfrancis.com
The first edition of the Textbook of Assisted Reproductive Techniques was published in
2001. As the textbook now enters its sixth edition, some 45 years since the birth of Louise …
2001. As the textbook now enters its sixth edition, some 45 years since the birth of Louise …