The identification of extracellular phospholipid vesicles as conveyors of cellular information
has created excitement in the field of drug delivery. Biological therapeutics, including short …

The native environment of membrane proteins is complex and scientists have felt the need
to simplify it to reduce the number of varying parameters. However, experimental problems …

Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique for structural
studies of chemical compounds and biomolecules such as DNA and proteins. Since the …

Membrane proteins frequently assemble into higher order homo-or hetero-oligomers within
their natural lipid environment. This complex formation can modulate their folding, activity as …

Cell-free expression has emerged as a powerful technique to overcome major restrictions of
classical in vivo membrane protein production, with sample yields of mgms of protein per ml …

Protein production using processed cell lysates is a core technology in synthetic biology and
these systems are excellent to produce difficult toxins or membrane proteins. However, the …

Cell-free protein production opens new perspectives for the direct manipulation of
expression compartments in combination with reduced complexity of physiological …

Despite advances in membrane protein (MP) structural biology and a growing interest in
their applications, these proteins remain challenging to study. Progress has been hindered …

Reversible membrane targeting of proteins is one of the key regulators of cellular interaction
networks, for example, for signaling and polarization. So-called “membrane switches” are …

The intensification of microreactions is important for biological and chemical reactions with
microliter volumes as reaction units, including microliter bioassay or protein synthesis. At the …