Phage liquid crystalline droplets form occlusive sheaths that encapsulate and protect infectious rod-shaped bacteria

AK Tarafder, A von Kügelgen… - Proceedings of the …, 2020 - National Acad Sciences
The opportunistic pathogen Pseudomonas aeruginosa is a major cause of antibiotic-tolerant
infections in humans. P. aeruginosa evades antibiotics in bacterial biofilms by up-regulating …

A multidomain connector links the outer membrane and cell wall in phylogenetically deep-branching bacteria

A von Kügelgen, S van Dorst, V Alva… - Proceedings of the …, 2022 - National Acad Sciences
Deinococcus radiodurans is a phylogenetically deep-branching extremophilic bacterium that
is remarkably tolerant to numerous environmental stresses, including large doses of …

Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus

M Herdman, B Isbilir, A von Kügelgen… - Nature …, 2024 - nature.com
Surface layers (S-layers) are proteinaceous, two-dimensional paracrystalline arrays that
constitute a major component of the cell envelope in many prokaryotic species. In this study …

[HTML][HTML] Complete atomic structure of a native archaeal cell surface

A von Kügelgen, V Alva, TAM Bharat - Cell Reports, 2021 - cell.com
Many prokaryotic cells are covered by an ordered, proteinaceous, sheet-like structure called
a surface layer (S-layer). S-layer proteins (SLPs) are usually the highest copy number …

[HTML][HTML] High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells

M Herdman, A von Kügelgen, D Kureisaite-Ciziene… - Structure, 2022 - cell.com
Surface layers (S-layers) are proteinaceous crystalline coats that constitute the outermost
component of most prokaryotic cell envelopes. In this study, we have investigated the role of …

The parasitic lifestyle of an archaeal symbiont

JN Hamm, Y Liao, A von Kügelgen… - Nature …, 2024 - nature.com
DPANN archaea are a diverse group of microorganisms characterised by small cells and
reduced genomes. To date, all cultivated DPANN archaea are ectosymbionts that require …

Compressed sensing for electron cryotomography and high-resolution subtomogram averaging of biological specimens

J Böhning, TAM Bharat, SM Collins - Structure, 2022 - cell.com
Cryoelectron tomography (cryo-ET) and subtomogram averaging (STA) allow direct
visualization and structural studies of biological macromolecules in their native cellular …

Plasmid-Encoded H-NS Controls Extracellular Matrix Composition in a Modern Acinetobacter baumannii Urinary Isolate

S Benomar, G Di Venanzio, MF Feldman - Journal of bacteriology, 2021 - Am Soc Microbiol
Acinetobacter baumannii is emerging as a multidrug-resistant (MDR) nosocomial pathogen
of increasing threat to human health worldwide. The recent MDR urinary isolate UPAB1 …

The intracellular lifestyle of an archaeal symbiont

JN Hamm, Y Liao, A von Kügelgen, N Dombrowski… - BioRxiv, 2023 - biorxiv.org
SUMMARY DPANN Archaea are a diverse archaeal clade characterised by small cells and
reduced genomes. To date, all cultivated DPANN Archaea are ectosymbionts that require …

HfaE is a component of the holdfast anchor complex that tethers the holdfast adhesin to the cell envelope

NK Chepkwony, GG Hardy, YV Brun - Journal of Bacteriology, 2022 - Am Soc Microbiol
Bacteria use adhesins to colonize different surfaces and form biofilms. The species of the
Caulobacterales order use a polar adhesin called holdfast, composed of polysaccharides …