The post-translational transport of cytoplasmically synthesized precursor proteins into
chloroplasts requires proteins in the envelope membranes. To identify some of these …

Three different extraction procedures for two‐dimensional electrophoresis of plant proteins
are compared:(i) extraction of soluble proteins with a nondenaturing Trisbuffer,(ii) denaturing …

A chloroplastic outer envelope membrane protein of 75 kDa (OEP75) was identified
previously as a component of the protein import machinery. Here we provide additional …

Several proteases, ie, pronase, a mixture of trypsin and chymotrypsin, and thermolysin were
screened as potential surface probes of isolated intact pea (Pisum sativum var Laxton's …

Publisher Summary This chapter discusses the isolation and characterization of chloroplast
envelope membranes. The chloroplast envelope consists of a pair of lipid bilayer …

Proteins from both the inner and outer envelope membranes are engaged in the recognition
and translocation of precursor proteins into chloroplasts. A 110 kDa protein of the …

Protein import into chloroplasts is powered by ATP hydrolysis in the stroma. Establishing the
identity and functional mechanism of the stromal ATPase motor that drives import is critical …

S-Adenosylmethionine (SAM) is formed exclusively in the cytosol but plays a major role in
plastids; SAM can either act as a methyl donor for the biogenesis of small molecules such as …

We introduce the use of Arabidopsis thaliana callus culture as a system for proteomic
analysis of plant organelles using liquid‐grown callus. This callus is relatively …

A membrane fraction enriched in chloroplast envelopes was isolated from the alga
Chlamydomonas reinhardii. The envelope preparations contained the envelope marker …