It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …

Several promising strategies based on combining or cycling different antibiotics have been
proposed to increase efficacy and counteract resistance evolution, but we still lack a deep …

Transfer RNAs (tRNAs) play a central role in protein translation. Studying them has been
difficult in part because a simple method to simultaneously quantify their abundance and …

The E4 allele of the apolipoprotein E gene (APOE) has been established as a genetic risk
factor for many diseases including cardiovascular diseases and Alzheimer's disease (AD) …

Several recent studies in a number of model systems including zebrafish, Arabidopsis, and
mouse have revealed phenotypic differences between knockouts (ie, mutants) and …

INTRODUCTION Genetic interactions occur when mutations in two or more genes combine
to generate an unexpected phenotype. An extreme negative or synthetic lethal genetic …

The genetic dependencies of human cancers widely vary. Here, we catalog this
heterogeneity and use it to identify functional gene interactions and genotype-dependent …

The genomes of malaria parasites contain many genes of unknown function. To assist drug
development through the identification of essential genes and pathways, we have measured …

Since the first genome-scale comparisons, it has been evident that the genomes of many
species are unbound by strict vertical descent: Large differences in gene content can occur …

Essential genes refer to genes that are required by an organism to survive under specific
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …