Chemistry, biology, and medicine of fluorescent nanomaterials and related systems: new insights into biosensing, bioimaging, genomics, diagnostics, and therapy

J Yao, M Yang, Y Duan - Chemical reviews, 2014 - ACS Publications
2. INTRODUCTION The investigation of many fundamental processes in life sciences
requires straightforward tools for fast, sensitive, reliable, and reproducible detection of …

Molecular biologist's guide to proteomics

PR Graves, TAJ Haystead - Microbiology and molecular biology …, 2002 - Am Soc Microbiol
The emergence of proteomics, the large-scale analysis of proteins, has been inspired by the
realization that the final product of a gene is inherently more complex and closer to function …

Current two‐dimensional electrophoresis technology for proteomics

A Görg, W Weiss, MJ Dunn - Proteomics, 2004 - Wiley Online Library
Abstract Two‐dimensional gel electrophoresis (2‐DE) with immobilized pH gradients (IPGs)
combined with protein identification by mass spectrometry (MS) is currently the workhorse …

[HTML][HTML] Two-dimensional gel electrophoresis in proteomics: a tutorial

T Rabilloud, C Lelong - Journal of proteomics, 2011 - Elsevier
Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of
proteomics. Although it is no longer the only experimental scheme used in modern …

Validation and development of fluorescence two‐dimensional differential gel electrophoresis proteomics technology

R Tonge, J Shaw, B Middleton… - PROTEOMICS …, 2001 - Wiley Online Library
Fluorescence two‐dimensional differential gel electrophoresis (2‐D DIGE) is a new
development in protein detection for two‐dimensional gels. Using mouse liver homogenates …

Visible fluorescent detection of proteins in polyacrylamide gels without staining

CL Ladner, J Yang, RJ Turner, RA Edwards - Analytical biochemistry, 2004 - Elsevier
2, 2, 2-Trichloroethanol (TCE) incorporated into polyacrylamide gels before polymerization
provides fluorescent visible detection of proteins in less than 5min of total processing time …

Unintended effects and their detection in genetically modified crops

F Cellini, A Chesson, I Colquhoun, A Constable… - Food and Chemical …, 2004 - Elsevier
The commercialisation of GM crops in Europe is practically non-existent at the present time.
The European Commission has instigated changes to the regulatory process to address the …

Peptide-based biopolymers in biomedicine and biotechnology

D Chow, ML Nunalee, DW Lim, AJ Simnick… - Materials Science and …, 2008 - Elsevier
Peptides are emerging as a new class of biomaterials due to their unique chemical,
physical, and biological properties. The development of peptide-based biomaterials is …

Detection technologies in proteome analysis

WF Patton - Journal of Chromatography B, 2002 - Elsevier
Common strategies employed for general protein detection include organic dye, silver stain,
radiolabeling, reverse stain, fluorescent stain, chemiluminescent stain and mass …

[HTML][HTML] 2D differential in-gel electrophoresis for the identification of esophageal scans cell cancer-specific protein markers

G Zhou, H Li, D DeCamp, S Chen, H Shu… - Molecular & Cellular …, 2002 - ASBMB
The reproducibility of conventional two-dimensional (2D) gel electrophoresis can be
improved using differential in-gel electrophoresis (DIGE), a new emerging technology for …