Nanodiscs in membrane biochemistry and biophysics

IG Denisov, SG Sligar - Chemical reviews, 2017 - ACS Publications
Membrane proteins play a most important part in metabolism, signaling, cell motility,
transport, development, and many other biochemical and biophysical processes which …

[HTML][HTML] To see or not to see: lateral organization of biological membranes and fluorescence microscopy

LA Bagatolli - Biochimica et Biophysica Acta (BBA)-Biomembranes, 2006 - Elsevier
In the last few years several experimental strategies based on epi-, confocal and two photon
excitation fluorescence microscopy techniques have been employed to study the lateral …

Daptomycin inhibits cell envelope synthesis by interfering with fluid membrane microdomains

A Müller, M Wenzel, H Strahl, F Grein… - Proceedings of the …, 2016 - National Acad Sciences
Daptomycin is a highly efficient last-resort antibiotic that targets the bacterial cell membrane.
Despite its clinical importance, the exact mechanism by which daptomycin kills bacteria is …

LXRs link metabolism to inflammation through Abca1-dependent regulation of membrane composition and TLR signaling

A Ito, C Hong, X Rong, X Zhu, EJ Tarling, PN Hedde… - elife, 2015 - elifesciences.org
The liver X receptors (LXRs) are transcriptional regulators of lipid homeostasis that also
have potent anti-inflammatory effects. The molecular basis for their anti-inflammatory effects …

Order of lipid phases in model and plasma membranes

HJ Kaiser, D Lingwood, I Levental… - Proceedings of the …, 2009 - National Acad Sciences
Lipid rafts are nanoscopic assemblies of sphingolipids, cholesterol, and specific membrane
proteins that contribute to lateral heterogeneity in eukaryotic membranes. Separation of …

Phasor S-FLIM: a new paradigm for fast and robust spectral fluorescence lifetime imaging

L Scipioni, A Rossetta, G Tedeschi, E Gratton - Nature methods, 2021 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) and spectral imaging are two broadly
applied methods for increasing dimensionality in microscopy. However, their combination is …

A selective membrane-targeting repurposed antibiotic with activity against persistent methicillin-resistant Staphylococcus aureus

W Kim, G Zou, TPA Hari, IK Wilt… - Proceedings of the …, 2019 - National Acad Sciences
Treatment of Staphylococcus aureus infections is complicated by the development of
antibiotic tolerance, a consequence of the ability of S. aureus to enter into a nongrowing …

Lpcat3-dependent production of arachidonoyl phospholipids is a key determinant of triglyceride secretion

X Rong, B Wang, MM Dunham, PN Hedde, JS Wong… - elife, 2015 - elifesciences.org
The role of specific phospholipids (PLs) in lipid transport has been difficult to assess due to
an inability to selectively manipulate membrane composition in vivo. Here we show that the …

[HTML][HTML] Two photon fluorescence microscopy of coexisting lipid domains in giant unilamellar vesicles of binary phospholipid mixtures

LA Bagatolli, E Gratton - Biophysical journal, 2000 - cell.com
Images of giant unilamellar vesicles (GUVs) formed by different phospholipid mixtures (1, 2-
dipalmitoyl-sn-glycero-3-phosphocholine/1, 2-dilauroyl-sn-glycero-3-phosphocholine …

Laurdan generalized polarization fluctuations measures membrane packing micro-heterogeneity in vivo

SA Sanchez, MA Tricerri… - Proceedings of the …, 2012 - National Acad Sciences
Cellular membranes are heterogeneous in composition, and the prevailing theory holds that
the structures responsible for this heterogeneity in vivo are small structures (10–200 nm) …