Background In the context of the current pandemic caused by the novel coronavirus,
molecular detection is not limited to the clinical laboratory, but also faces the challenge of …

Cystic fibrosis (CF) is a life-threatening autosomal-recessive disease caused by mutations in
a single gene encoding cystic fibrosis transmembrane conductance regulator (CFTR). CF …

Harnessing the bacterial clustered regularly interspaced short palindromic repeats
(CRISPR) system for genome editing in eukaryotes has revolutionized basic biomedical …

At present, the main challenge for Staphylococcus aureus (S. aureus) in food safety is still a
lack of rapid, convenient detection methods. This study developed a combination of …

Variable industrial strains have been applied in the fermentation of bulk amino acids. Strain
discovery and evolution, process optimization are traditional approaches to improve the …

Adaptive laboratory evolution (ALE) is a widely used and highly effective tool for improving
microbial phenotypes and investigating the evolutionary roots of biological phenomena …

The CRISPR/Cas system has gained enormous attention for its excellent gene-editing
capabilities. In recent years, the reported trans-cleavage activity of some Cas proteins …

Gene drive is the process of copying of an endonuclease‐containing cassette that leads to
increased frequency of inheritance of the desired traits in a targeted population …

The lung has long been a target for gene therapy, yet efficient delivery and phenotypic
disease correction has remained challenging. Although there have been significant …

Transgenic animals with increased or abrogated target gene expression are powerful tools
for drug discovery research. Here, we developed a CRISPR-based Rosa26-LSL-dCas9 …