The formation of vascular lumens by endothelial cells is a critical step in the angiogenic
process that occurs during invasion and growth of the incipient vascular sprout. Once a …

Long-term viability of thick three-dimensional engineered tissue constructs is a major
challenge. Addressing it requires development of vessel-like network that will allow the …

Despite extensive research, pro-angiogenic drugs have failed to translate clinically, and
therapeutic angiogenesis, which has potential in the treatment of various cardiovascular …

When suspended in collagen gels, endothelial cells elongate and form capillary-like
networks containing lumens. Human blood outgrowth endothelial cells (HBOEC) suspended …

During angiogenesis, endothelial cells initiate a tissue-invasive program within an interstitial
matrix comprised largely of type I collagen. Extracellular matrix–degradative enzymes …

The migration of endothelial cells (ECs) plays an important role in vascular remodeling and
regeneration. EC migration can be regulated by different mechanisms such as chemotaxis …

This protocol describes how to form a 3D cell culture with explicit, endothelialized
microvessels. The approach leads to fully enclosed, perfusable vessels in a bioremodelable …

The endothelial cell (EC)–derived tissue inhibitor of metalloproteinase-2 (TIMP-2) and
pericyte-derived TIMP-3 are shown to coregulate human capillary tube stabilization …

A class of designed self-assembling peptide nanofiber scaffolds with more than 99% water
content has been shown to be a good biological material for cell culture. Here, we report the …

A significant stumbling block in the creation of functional three-dimensional (3D) engineered
tissues is the proper vascularization of the constructs. Furthermore, in the context of …