Engineering microbial cells to efficiently synthesize high-value-added natural products has
received increasing attention in recent years. In this review, we describe the pipeline to build …

Abstract The trisaccharide, 2′-fucosyllactose (Fucα1-2Galβ1-4Glc; 2′-FL), is the most
abundant oligosaccharide in human milk. It has numerous significant biological properties …

Highlights•Recent advances for microbial HMO synthesis are described.•Key challenges in
representative HMO production are highlighted.•Further perspectives in general HMO …

The cryptic secondary metabolite biosynthetic gene clusters (BGCs) far outnumber currently
known secondary metabolites. Heterologous production of secondary metabolite BGCs in …

Fucosyllactose (2′-FL) is a kind of fucosylated lactose of human milk oligosaccharides
(HMOs). In this work, Escherichia coli MG1655 was metabolically engineered to increase …

Human milk oligosaccharides (HMOs), a group of structurally diverse unconjugated glycans
in breast milk, act as important prebiotics and have plenty of unique health effects for …

2′-Fucosyllactose (2′-FL) is the most abundant oligosaccharide in human milk. In this
study, a highly efficient biosynthetic route for 2′-FL production was designed via the de …

Food is essential to provide energy for human cellular metabolism, and is usually made from
plants or animals. Beside plants and animals, other important food sources are made by …

Background The most abundant human milk oligosaccharide in breast milk, 2′-
fucosyllactose (2′-FL), has been approved as an additive to infant formula due to its …

Human milk oligosaccharides (HMO) have attracted great interest in recent years due to
their role in boosting infants and adults health. According to several in vitro, in vivo and …